化工学报2025，Vol.76Issue(3)：922-932,11.DOI:10.11949/0438-1157.20240895
CRISPR技术在链霉菌细胞工厂中的应用和优化
Application and optimization of CRISPR editing technology in Streptomyces
摘要
Abstract
Streptomyces chassis development is an effective strategy for the high-efficient synthesis of microbial source nature product,and highly efficient gene editing tools provide a powerful tool for the construction of Streptomyces cell factories.CRISPR(clustered regularly interspaced short palindromic repeats)technology is accurate,universal and easy to operate,and has been widely used in microorganisms.However,off-target effects,cytotoxicity and low editing efficiency limit application of CRISPR genome editing in Streptomyces.This article summarizes the application and optimization strategies of CRISPR-related technologies in Streptomyces,and looks forward to the application prospects of CRISPR systems in Streptomyces cell factories,providing a reference for the development of efficient gene editing tools suitable for Streptomyces.关键词
CRISPR/链霉菌/基因编辑/生物技术/优化策略Key words
CRISPR/Streptomyces/gene editing/biological techniques/optimization分类
生物工程引用本文复制引用
李新颖,苏畅,郭超,庞建,王超,李春..CRISPR技术在链霉菌细胞工厂中的应用和优化[J].化工学报,2025,76(3):922-932,11.基金项目
国家自然科学基金项目(22108153) （22108153）
十四五重点研发计划项目(2023YFA0914700) （2023YFA0914700）
工业生物催化教育部重点实验室(清华大学)开放基金项目(2023003) （清华大学）
