华西口腔医学杂志2025，Vol.43Issue(2)：183-189,7.DOI:10.7518/hxkq.2025.2024392
微阵列技术探究限域面积对成牙本质细胞极化和分化的影响
Effects of cell area on single odontoblast polarization and differentiation via microarray technology
摘要
Abstract
Objective This study aimed to explore the impact of cell spreading area on odontoblast polariza-tion and differentiation using micropatterned surfaces ge-nerated by photolithography.Methods Micropatterned surfaces with differential adhesive properties were pre-pared using polyethylene glycol diacrylate(PEGDA)-ba-sed photolithography.Human dental pulp stem cells(hD-PSCs)were isolated into single cells and cultured on micropatterned surfaces with areas of 1 800,2 700,and 3 600 μm2.Immunofluorescence staining was used to observe cell morphology and analyze the relocating of the golgi apparatus and nucleus.Alkaline phosphatase staining was preformed to examine odontogenic differentiation.Results The hDPSCs were successfully isolated and cultured on micropatterned surfaces mimicking the morphology of polarized odontoblasts.Phalloidin staining confirmed that the isolated hDPSCs successfully recapitulated the morphology of predesigned mi-cropatterns.Immunofluorescence staining showed that the polarization and differentiation levels of the hDPSCs with a 3600 μm2 area were significantly higher than those with 1 800 and 2 700 μm2 areas(P<0.05).Conclusion The polariza-tion and differentiation of single hDPSCs increased with the cell areas on micropatterned surfaces.关键词
细胞极化/图案化/成牙本质细胞/细胞分化Key words
cell polarization/micropatterning/odontoblast/cell differentiation分类
口腔医学引用本文复制引用
李虎恩,于年祚,李熙恒,唐晓铎,孙雅鹿,司超,张俊虎,常蓓..微阵列技术探究限域面积对成牙本质细胞极化和分化的影响[J].华西口腔医学杂志,2025,43(2):183-189,7.基金项目
Natural Science Foundation of China(82101075) （82101075）
Young Elite Scientists Sponsorship Program by China Association for Science and Technology(YESS20210407) （YESS20210407）
China Postdoctoral Science Foundation(2022M711301) （2022M711301）
Bet-hune Project of Jilin University(419161923025) 国家自然科学基金青年基金(82101075) （419161923025）
中国科学技术协会青年人才托举项目(YESS20210407) （YESS20210407）
博士后科学基金面上项目(2022M711301) （2022M711301）
吉林大学"白求恩计划"(419161923025) （419161923025）
