华西口腔医学杂志2025，Vol.43Issue(2)：236-248,13.DOI:10.7518/hxkq.2025.2024393
人参皂苷Rb3调节磷酸化细胞外信号调节激酶通路减轻牙周炎大鼠炎症反应促进成骨
Ginsenoside Rb3 regulates the phosphorrylated extracellular signal-regulated kinase signaling pathway to allevi-ate inflammatory responses and promote osteogenesis in rats with periodontitis
摘要
Abstract
Objective To explore the promoting effect of ginsenoside Rb3(Rb3)on osteogenesis in periodontitis environment,and to explain its mechanism.Methods Human periodontal ligament stem cells(hPDLSCs)were cul-tured by tissue block method and identified by flow cytometry.Cell counting kit-8(CCK8)method and calcein ace-toxymethyl ester/propidium iodide staining were used to detect the effect of Rb3 on the viability of hPDLSCs cells.In vitro cell experiments were divided into control group,10 μg/mL lipopolysaccharides(LPS)group,10 μg/mL LPS+100 μmol/L Rb3 group and 10 μg/mL LPS+200 μmol/L Rb3 group.Alkaline phosphatase(ALP)staining was used to detect the ALP activity of hPDLSCs in each group after osteogenesis induction.The expression of hPDLSCs interleukin-6(IL-6),interleukin-8(IL-8),runt-related transcription factor 2(RUNX2)and transforming growth fac-tor-β(TGF-β)genes in each group after osteogenesis was detected by quantitative reverse transcription polymerase chain reaction(qRT-PCR)method.Western blot was used to detect the protein expression of hPDLSCs phosphorrylat-ed extracellular signal-regulated kinase(p-ERK)in each group.Sprague-Dawley rats were randomly divided into the control group,ligation group and ligation+Rb3 group.The left molar-maxillary tissue was subjected to micro-comput-ed tomography(micro-CT)scanning.After the scanning,the left molar-maxilla was made into periodontal tissue sec-tions.Hematoxylin-eosin(HE)staining was used to detect the infiltration and loss of adhesion of inflammatory cells.Masson staining was used to detect the destruction of gingival collagen fibers.Immunofluorescence staining was used to detect the protein expression of RUNX2 and p-ERK.The expression of TGF-β in rat gingival tissue was de-tected by qRT-PCR.The protein expression of IL-6 in peripheral serum of rats was detected by enzyme-linked immu-nosorbent assay(ELISA).Flow cytometry was used to detect the proportion of Treg cells in rat heart blood.The ex-perimental data were statistically analyzed by Graph Pad Prism10.1.2 software.Results Rb3 had no effect on the cell activity of hPDLSCs.The results of qRT-PCR and ALP staining showed that Rb3 could inhibit the gene expres-sion of IL-6 and IL-8 in inflammatory hPDLSCs,promote TGF-β gene and promote the osteogenic differentiation of inflammatory hPDLSCs.Western blot showed that Rb3 inhibited the protein expression of inflammatory hPDLSCs p-ERK.The results from micro-CT,Masson staining,and HE staining demonstrated that Rb3 promotes alveolar bone formation in rats with periodontitis,while simultaneously inhibiting the destruction of periodontal fibrous tissue,re-ducing attachment loss,and suppressing inflammatory cell infiltration.The results of flow cytometry showed that Rb3 could promote the differentiation of Treg cells in peripheral blood of periodontitis rats.The results of ELISA and qRT-PCR showed that Rb3 could inhibit the protein expression of IL-6 and promote the gene expression of TGF-β in periodontitis rats.Immunofluorescence results showed that Rb3 could promote the protein expression of RUNX2 and inhibit the protein expression of p-ERK in periodontitis rats.Conclusion Rb3 can reduce the inflammatory reaction of periodontal tissues in periodontitis rats,and promote the osteogenic differentiation of hPDLSCs by regulating p-ERK pathways.关键词
人参皂苷Rb3/磷酸化细胞外信号调节激酶/成骨分化/牙周炎/牙槽骨吸收Key words
ginsenoside Rb3/phosphorrylated extracellular signal-regulated kinase/osteogenic differentiation/periodontitis/alveolar bone resorption分类
医药卫生引用本文复制引用
张雪颖,孟鑫,刘志臻,张康,冀洪海,孙敏敏..人参皂苷Rb3调节磷酸化细胞外信号调节激酶通路减轻牙周炎大鼠炎症反应促进成骨[J].华西口腔医学杂志,2025,43(2):236-248,13.基金项目
山东省自然科学基金青年项目(ZR2022QH273) National Natural Science Foundation of Shandong(ZR2022QH273) （ZR2022QH273）
