经济动物学报2025，Vol.29Issue(1)：68-74,7.DOI:10.13326/j.jea.2025.2030
1株犬疱疹病毒的分离与鉴定
Isolation and Identification of A Strain of Canine Herpesvirus
摘要
Abstract
Canine herpesvirus(CHV)has a high infection and mortality rate in newborn puppies,causing serious economic losses to the dog breeding industry.Currently,there are few reports on this virus,and there is a lack of effective vaccines for prevention and control of the virus infection.A strain of the virus was isolated from sample collected from a sick dog using Madin-Darby Canine Kidney(MDCK)cells,which named as CHV-202402.The isolated strain was identified through methods such as observation of viral particle morphology,indirect immunofluorescence(IFA)assay,Western blot,and homology analysis of gene sequences.The results showed that the CHV-202402 strain could produce typical CPE on MDCK cells,with a viral titer of 10-4 TCID50/0.1 mL at the third passage;spherical,enveloped viral particles could be observed under the electron microscope;IFA and Western blot results indicated that the isolated strain could specifically bind with the monoclonal antibodies against CHV;homology analysis of the gD gene nucleotide sequence showed that it was highly homologous to that of domestic and international strains.These results proved that the isolated strain was the canine herpesvirus.The successful solation of a CHV strain has enriched the genomic data of CHV and laid the foundation for studying the characteristics of CHV and establishing related detection methods.关键词
犬疱疹病毒/分离/鉴定/gD基因Key words
canine herpesvirus/isolation/identification/gD gene分类
畜牧业引用本文复制引用
张子晨,蔡霖颖,樊益铭,唐净杰,杜庆庆,朱英奇,王桂军,刘光清..1株犬疱疹病毒的分离与鉴定[J].经济动物学报,2025,29(1):68-74,7.基金项目
国家重点研发计划项目(2023YFD1800700),中国博士后科学基金项目(2024M753586),安徽省高校协同创新项目(GXXT-2022-057) （2023YFD1800700）
