解剖学杂志2025，Vol.48Issue(1)：7-11,封2,6.DOI:10.3969/j.issn.1001-1633.2025.01.002
低氧环境对妊娠大鼠胎盘组织TNF-α、IL-10的表达及其生殖功能、胚胎发育的影响
Effects of hypoxia on expression of TNF-α and IL-10 in placental tissue,reproductive function and embryonic development of pregnant rats
摘要
Abstract
Objective:To investigate the expression of tumor necrosis factor-alpha(TNF-α)and interleukin-10(IL-10)in the placenta under high-altitude hypoxic conditions,and the effects on reproductive function and offspring development.Methods:A total of 20 female rats and 10 male rats were randomly divided into hypoxic group(simulated 6 000 m altitude in a hypobaric oxygen chamber)and normoxia group(Xining city,Qinghai province,with an average altitude of 2 200 m)with a female-to-male ratio of 2:1 per cage.The pregnancy rate of female rats in each group was recorded.On the 20th day of gestation,after a 12-hour fast,blood samples were collected from the tails of the pregnant rats to measure their fasting glucose.Blood was also taken from the abdominal aorta,and the entire uterus was excised to isolate the placenta and fetuses.The uterine weight and number of fetal rats were recorded,and the offspring malformation rate was calculated for both groups.ELISA was used to detect the serum fasting insulin level of pregnant rats,and H-E staining was used to observe the alteration of placental tissues in hypoxia.Immunohistochemistry was utilized to detect the positive expression of TNF-α and IL-10 in the placental tissues of pregnant rats.RT-qPCR and Western blotting were employed to detect the mRNA and protein expression of TNF-α and IL-10 in placental tissues of pregnant rats.Results:Compared with the normoxia group,the hypoxia group showed significantly lower uterine weight in pregnant rats during late pregnancy,reduced fasting blood glucose and increased fasting insulin levels,lower conception rates,fewer fetuses,and a higher rate of fetal malformations.The placenta of pregnant rats in the hypoxia group was structurally incomplete,with increased distribution of blood vessels,thickened vessel wall,slow development and reduced number of chorionic villi,densely distributed intervillous blood vessels,thickened basement membrane,a small number of inflammatory cells,and blurred boundaries of thickened syncytial trophoblast.Compared with the normoxia group,the expression of TNF-α and IL-10 mRNA and protein were up-regulated in the placental tissues of the hypoxia group.Conclusion:Under hypoxic conditions,the expression of TNF-α and IL-10 in the placental tissue is upregulated,which may be related to impaired reproductive function and adverse pregnancy outcomes in female rats.关键词
高原低氧/妊娠大鼠/肿瘤坏死因子α/白介素10/生殖功能/胎盘Key words
plateau hypoxia/pregnant rats/tumor necrosis factor-α/interleukin-10/reproductive function/placenta分类
基础医学引用本文复制引用
李丹,李建华,许佳佳..低氧环境对妊娠大鼠胎盘组织TNF-α、IL-10的表达及其生殖功能、胚胎发育的影响[J].解剖学杂志,2025,48(1):7-11,封2,6.基金项目
青海省科技厅项目(2019-SF-134) （2019-SF-134）
