解剖学杂志2025，Vol.48Issue(1)：12-16,33,6.DOI:10.3969/j.issn.1001-1633.2025.01.003
转录因子AP-1参与孕酮下调蜕膜基质细胞IL-8表达对早期自然流产的影响
Transcription factor AP-1 mediates progesterone-induced downregulation of IL-8 expression in decidual stromal cells and its effect on early spontaneous abortion
摘要
Abstract
Objective:To explore the regulatory effect of progesterone on the expression of interleukin-8(IL-8)in decidual stromal cells(DSCs)through transcription factor activator protein 1(AP-1).Methods:Immunohistochemical staining and western blotting(WB)were used to detect the expression level of AP-1 protein in decidual tissue of the abortion group and the control group.Early pregnancy human DSCs were isolated and cultured in vitro,and cells were treated with different concentrations of progesterone(0.01 μmol/L,0.1 μmol/L,and 1 μmol/L).WB was used to detect the expression of AP-1 and p-AP-1 in DSCs.After AP-1 inhibitor SR-11302 treatment,the expression of IL-8 in the inhibitor group,progesterone group,and control group was detected by using RT-qPCR and WB.Results:The expression level of p-AP-1 protein in decidual tissue of the abortion group was higher than that of the control group;the relative expression levels of p-AP-1 protein in the 0.1 μmol/L and 1 μmol/L progesterone group were significantly lower than that in the control group;IL-8 mRNA levels were significantly lower in the progesterone group compared with the control group,and were further reduced in the progesterone+SR-11302 group compared with the progesterone group;compared with the control group,the relative expression levels of IL-8 and p-AP-1 proteins in the progesterone group were reduced,and compared with the progesterone group,the relative expression levels of IL-8 and p-AP-1 proteins in the progesterone+SR-11302 group were also reduced.Conclusions:Progesterone downregulates the expression of IL-8 in DSCs via AP-1,offering a theoretical basis for its use in preventing and treating early spontaneous abortion induced by abnormal expression of proinflammatory cytokines.关键词
激活蛋白1/白细胞介素-8/蜕膜基质细胞/孕酮/早期自然流产Key words
activator protein-1/interleukin 8/decidual stromal cells/progesterone/early spontaneous abortion分类
基础医学引用本文复制引用
宋芳,李永红,李娜,栾兆进,赵紫薇,宫晓玲,杨美霞..转录因子AP-1参与孕酮下调蜕膜基质细胞IL-8表达对早期自然流产的影响[J].解剖学杂志,2025,48(1):12-16,33,6.基金项目
内蒙古自治区自然科学基金(2021MS08056,2022MS08037,2023MS08063) （2021MS08056,2022MS08037,2023MS08063）
内蒙古自治区卫生健康科技计划项目(202201393) （202201393）
包头医学院博士启动基金(BSJJ202205) （BSJJ202205）
包头医学院创新团队发展计划项目(bycxtd-03) （bycxtd-03）
