摘要
Abstract
Objective:To investigate the effect of tyrosine phosphatase SHP2 in mesenchymal stem cells(MSCs)on osteoporosis.Method:Forty mice were evenly divided into sham operation group,osteoporosis model group,osteoporosis model+MSCs group,and osteoporosis model+MSCs+SHP2 shRNA group.A dual energy X-ray absorptiometer was used to detect mouse bone density.The trabecular bone tissue of mice was stripped,stained with Hematoxylin and Eosin(H-E)staining,observed under a microscope,and photographed for records.In vitro experiments,MSCs were divided into NC group,bone morphogenetic protein 2(BMP-2)group,BMP2+SHP2 mimic group,BMP2 stimulation+SHP2 mimic group.CCK-8 and colony formation assays were conducted.Western blotting was used to detect the expression of SHP2,ERK,and cyclin D1 proteins in MSCs cells.Additionally,Western blotting was used to detect osteoblast markers in MSCs.Result:Osteoporotic mice treated with MSCs showed significant improvement in their condition.The H-E staining results showed that the bone trabeculae in the sham group were normal,while those in the Model group were extremely poor.The bone trabeculae in the Model+MSCs group showed a significant increase compared with the Model group.The bone trabeculae in the Model+MSCs+SHP2 shRNA group was worse than those in the Model+MSCs group.CCK-8 and colony formation assays showed that the NC group had less proliferation of MSCs,while the BMP2 stimulation group had more cell proliferation.The BMP2 stimulation+SHP2 shRNA group had slightly less cell proliferation than the BMP2 stimulation group.After adding BMP2,the shRNA transfection of SHP2 in MSCs can inhibit the expression of ERK,cyclin D1 and osteoblast markers.SHP2 in MSCs was transfected with mimic can promote the expression of ERK,cyclin D1,and osteoblast markers.Conclusion:Tyrosine phosphatase SHP2 in MSCs can promote MSCs cell proliferation and treat osteoporosis by activating the ERK/cycli D1 signaling pathway.关键词
间充质干细胞/SHP2/ERK/cyclin D1信号通路/骨质疏松Key words
mesenchymal stem cells/SHP2/ERK/cycli D1 signaling pathway/osteoporosis分类
基础医学
