解剖学杂志2025，Vol.48Issue(1)：23-27,5.DOI:10.3969/j.issn.1001-1633.2025.01.005
汉黄芩苷对高脂饮食诱导的糖尿病大鼠肝损伤的保护作用
Protective effect of Wogonoside on hepatic injury induced by high-fat diet in diabetic rats
摘要
Abstract
Objective:To investigate the protective effect and mechanism of Wogonoside(WO)on hepatic injury induced by high-fat diet in diabetic rats.Methods:A total of 50 male Wistar rats were randomly divided into control group,model group(DM)and three drug administration groups(DM+WO 2,4 and 8 mg/kg).The diabetic rat model was established by intraperitoneal injection of 30 mg/kg streptozotocin.Total cholesterol(TC),alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),superoxide dismutase(SOD),and malondialdehyde(MDA)were detected by ELISA.The expression levels of apoptosis-related proteins and lipid metabolism-related proteins in liver tissues were determined by Western blotting.H-E staining was used to observe the histopathological changes of liver.Apoptosis of hepatocytes was assessed by TUNEL staining.Results:Compared with the control group,the rats in the DM group showed typical symptoms of diabetes(frequent urination,thirst,hunger,and weight loss)and hepatic pathological injury.Compared with the DM group,the levels of biochemical indicators such as AST,ALT,TC,TG,LDL-C and MDA were obviously decreased in the drug administration groups,while the levels of HDL-C and SOD were obviously increased.The Bax/Bcl-2 ratio and acetyl-CoA carboxylase expression in liver tissue were significantly reduced,while the expression levels of c-Myc and carnitine palmitoyltransferase 1 were significantly elevated in the drug administration groups.The histopathological morphology of the liver was improved and the apoptosis of liver cells was reduced in the three WO treatment group,among which only a small number of apoptotic cells were observed in DM+WO 8 mg/kg group.Conclusion:WO has a certain protective effect on liver injury induced by high-fat diet in diabetic rats.关键词
汉黄芩苷/糖尿病/肝损伤/谷草转氨酶/凋亡Key words
Wogonoside/diabetes mellitus/hepatic injury/aspartate aminotransferase/apoptosis分类
基础医学引用本文复制引用
袁月,王丹鹤,李淑静,仵玉玲,董欣..汉黄芩苷对高脂饮食诱导的糖尿病大鼠肝损伤的保护作用[J].解剖学杂志,2025,48(1):23-27,5.基金项目
北京市自然科学基金青年项目(7184214) （7184214）
