解剖学杂志2025，Vol.48Issue(1)：28-33,6.DOI:10.3969/j.issn.1001-1633.2025.01.006
白桦脂酸通过抑制TGF-β2抑制A2780卵巢癌细胞上皮间质转化
Betulinic acid inhibits epithelial-mesenchymal transition in A2780 ovarian cancer cells by suppressing TGF-β2
摘要
Abstract
Objective:To investigate the effect and mechanism of betulinic acid(BA)on epithelial-mesenchymal transition(EMT)in A2780 ovarian cancer cells.Methods:The viability of A2780 cells treated with different concentrations of betulinic acid(5-80 μmol/L)for 24 hours was assessed by using the CCK-8 assay.The effects of BA on epithelial-mesenchymal transformation were detected under treatment with 0,5,10,20 μmol/L of BA for 24 h.The transforming growth factor-β(TGF-β)subtype involved in ovarian cancer was predicted by Oncomine,and the mRNA levels of TGF-β2 in A2780 cells treated with BA(0,5,10,20 µmol/L)for 24 hours were measured.A2780 cells were divided into control group,10 μmol/L BA group,and 10 μmol/L BA+2 ng/mL TGF-β2 group to determine whether TGF-β2 could reverse the effect of BA on epithelial mesenchymal transformation,invasion,migration and drug resistance in A2780 cells.Results:BA decreased the cell viability of A2780 with an IC50 of 37.8 μmol/L.Compared with 0 μmol/L BA group,the expression of E-cadherin was increased,while N-cadherin,Vimentin were significantly decreased in the 10 μmol/L and 20 μmol/L BA groups.Oncomine prediction results showed significant differences in both DNA copy numbers and mRNA expression levels of TGF-β2 between ovarian cancer and normal tissue.Compared with 0 μmol/L BA group,the expression of TGF-β2 were significantly decreased in the 5,10,20 μmol/L BA groups.Compared with the 10 μmol/L BA group,the 10 μmol/L BA+2 ng/mL TGF-β2 group had decreased E-cadherin,increased protein expression of N-cadherin,Vimentin,MMP-2,MMP-9,Smad2,Smad3,along with increased number of invaded cells,enhanced wound healing rate,and improved viability of cisplatin-treated cells.Conclusion:BA may exert its anti-tumor effects by inhibiting the TGF-β2-associated EMT signaling,thereby reducing the invasion,migration,and drug resistance of A2780 ovarian cancer cells.关键词
白桦脂酸/卵巢癌/转化生长因子β/上皮间质转化/转移Key words
betulinic acid/ovarian cancer/transforming growth factor-β/epithelial-mesenchymal transition/metastasis分类
基础医学引用本文复制引用
黄岳,张翔,庞学成,高洁凡,颜利求..白桦脂酸通过抑制TGF-β2抑制A2780卵巢癌细胞上皮间质转化[J].解剖学杂志,2025,48(1):28-33,6.基金项目
河北省重点研发计划项目(172777163) （172777163）
