摘要
Abstract
Objective:To investigate the effects of puerarin on the growth,proliferation,apoptosis and invasion of ovarian cancer Caov3 cells by regulating tumor immune microenvironment.Methods:Caov3 cells were treated with supernatants from THP-1 cells treated with different concentrations of puerarin.CCK8 assay was used to detect the growth of ovarian cancer cells.ELISA was utilized to detect the content of interleukin(IL)4,IL-10,and IL-6.RT-PCR was adopted to detect the mRNA expression of IL-4,IL-10,and IL-6.Colony formation assay was adopted to detect the number of cell colonies.Flow cytometry was applied to detect cell apoptosis.Western blotting was adopted detect the expression of apoptosis marker proteins(caspase-3,caspase-9).Transwell chamber was employed detect cell invasion.Western blotting was used to detect the expression of invasion-related proteins(vascular endothelial growth factor,fibronectin,vimentin).Results:Compared with the control group,when the puerarin concentration was 10 μmol/L,the viability of ovarian cancer Caov3 cells was significantly inhibited.Compared with the 0 μmol/L group,the levels and mRNA expression of IL-6,as well as the apoptosis rate,were significantly increased,while clone formation rate,number of invasion cells,and invasion-related protein expression were all significantly reduced.The results for IL-4 and IL-10 were opposite.Conclusion:Puerarin modulates tumor immune microenvironment to inhibit the growth and invasion of ovarian cancer Caov3 cells and to promote their apoptosis,thereby alleviating the occurrence and development of ovarian cancer tumors.关键词
葛根素/卵巢癌/Caov3细胞/侵袭/血管内皮生长因子Key words
puerarin/ovarian cancer/Caov3 cells/invasion/vascular endothelial growth factor分类
基础医学
