摘要
Abstract
Objective:To investigate the effect of long non-coding RNA(LncRNA)Down syndrome cell adhesion molecule antisense 1(DSCAM-AS1)on liver cancer activity and chemotherapy resistance by regulating the miR-431-5p/sex determining gene box 9(SOX9)axis.Methods:qRT-PCR was applied to detect the expression levels of DSCAM-AS1,miR-431-5p,and SOX9 mRNA in liver cancer tissue,liver cancer cells,and HepG2/CDDP resistant cells.Western blotting was utilized to detect the expression of multidrug resistance associated protein 1(MRP1),proliferating cell nuclear antigen(PCNA),Bcl-2,Bax,SOX9.Functional assays included MTT for cell viability,flow cytometry for apoptosis.Bioinformatics,dual luciferase assay,pull down assay,and RIP assay were applied to validate the targeting relationship between DSCAM-AS1,SOX9,and miR-431-5p.Results:DSCAM-AS1 and SOX9 were highly expressed in liver cancer tissues and cells,while miR-431-5p was low expressed.compared with HepG2 cells,the expression of DSCAM-AS1 and SOX9,and the cell viability in HepG2/CDDP cells were significantly increased,while the expression of miR-431-5p was reduced.Silencing DSCAM-AS1 inhibited the proliferation of HepG2 and HepG2/CDDP cells,induced apoptosis,and reduced drug resistance of HepG2/CDDP cells,while inhibiting miR-431-5p weakened the inhibitory effect of silencing DSCAM-AS1 on HepG2 and HepG2/CDDP cell proliferation,promoted apoptosis,and promoted drug resistance in HepG2/CDDP cells.Bioinformatics,dual luciferase assay,pull down assay,and RIP assay confirmed the targeting relationship between DSCAM-AS1,SOX9,and miR-431-5p.Conclusion:Silencing DSCAM-AS1 may inhibit HepG2 cell proliferation,promote apoptosis,and reduce drug resistance in HepG2/CDDP cells by regulating the miR-431-5p/SOX9 axis.关键词
肝癌/化疗耐药性/长链非编码RNA唐氏综合征细胞黏附分子反义1/miRNA-431-5p/性别决定基因盒9Key words
liver cancer/chemotherapy resistance/long non-coding RNA down syndrome cell adhesion molecule 1/miRNA-431-5p/sex determining gene box 9分类
基础医学
