西南医科大学学报2025，Vol.48Issue(2)：177-183,7.DOI:10.3969/j.issn.2096-3351.2025.02.011
盐酸丁卡因诱导的巨噬细胞转录组变化及可变剪接事件分析
Analysis of Transcriptome Changes and Alternative Splicing Events Induced by Tetracaine Hydrochloride in Macrophages
摘要
Abstract
Objective To investigate the effects of tetracaine hydrochloride on gene expression profiles and RNA alternative splicing patterns in macrophages.Methods In this study,we utilized transcriptome sequencing technology to analyze mouse macro-phage RAW 264.7 cells treated with 200 µM tetracaine hydrochloride for 24 hours.Differentially expressed mRNAs showing changes greater than two-fold were identified and analyzed using bioinformatics tools for GO functional categorization,KEGG pathway enrich-ment,Reactome pathway analysis,and differential alternative splicing events analysis.Results After tetracaine hydrochloride treat-ment of RAW 264.7 cells,a total of 52 119 differentially expressed genes were identified,including 1 785 upregulated and 1 454 down-regulated genes.GO analysis indicated significant enrichment in biological processes involved in DNA unwinding,DNA replication,T cell chemotaxis,DNA replication initiation,and DNA duplex unwinding.KEGG pathway analysis revealed enrichment in pathways such as Ribosome,Coronavirus disease-COVID-19,Oxidative phosphorylation,Cell cycle,p53 signaling pathway,and animal Mitophagy.Reactome analysis showed enrichment in processes such as Nonsense Mediated Decay(NMD)independent of the Exon Junction Complex(EJC),SRP-dependent co-translational protein targeting to membrane,and rRNA processing.GSEA analysis indi-cated activation of immune-inflammatory responses and the p53 pathway,while the cell cycle pathway was suppressed.Differential alternative splicing analysis indicated that tetracaine treatment significantly affected alternative splicing events in macrophages.Con-clusion The local anesthetic tetracaine hydrochloride significantly affected the gene expression profile of macrophages.The differen-tially expressed genes were closely related to DNA damage repair,cell cycle,immune response,ribosomal RNA processing,and nonsense-mediated RNA decay.Additionally,tetracaine also significantly influenced the alternative splicing process in macrophages.关键词
局麻药/盐酸丁卡因/巨噬细胞/转录组/差异基因Key words
Local anesthetic/Tetracaine hydrochloride/Macrophage/Transcriptome/Differential gene分类
医药卫生引用本文复制引用
秦正山,张燃,王唯一,赵鑫,易鹏,冯建国..盐酸丁卡因诱导的巨噬细胞转录组变化及可变剪接事件分析[J].西南医科大学学报,2025,48(2):177-183,7.基金项目
泸州市人民政府-西南医科大学科技战略合作项目(2021LZXNYD-D08) （2021LZXNYD-D08）
四川省大学生创新创业训练计划项目(S202310632238) （S202310632238）
