木材科学与技术2025，Vol.39Issue(1)：64-70,7.DOI:10.12326/j.2096-9694.2024076
高效液相色谱法测定防腐材中嘧菌酯含量的方法研究
Measurement of the Azoxystrobin Content in Preservative-Treated Wood by High Performance Liquid Chromatography
摘要
Abstract
In order to measure the content of azoxystrobin in treated wood precisely,a high performance liquid chromatography(HPLC)analysis method was constructed using a UV detector and C18 reversed-phase chromatography column,the V(methanol)∶V(water)=3∶1 as the mobile phase,with a flow rate of 0.8 mL/min and UV detection wavelength of 220 nm.Then,the ultrasonic extraction was used for pre-treatment of preservative extraction in wood/bamboo,and the extraction conditions were optimized.The results showed that the determination coefficient of the standard curve for azoxystrobin was 0.999 7 within the mass concentration range of 10～120 mg/L,and the linear correlation was significant.The coefficients of variation were 1.39%and 1.55%,and the recovery rates were 96.5%～101.6%.Under the optimized ultrasonic extraction conditions,including the solvent ratio of v(methanol)∶v(water)=8∶2,the extraction time of 60 minutes,and two retention levels,the azoxystrobin recovery rate of Radiata pine,masson pine,Chinese fir,and poplar'107'was 95.5%～99.6%,with a standard deviation of 0.8%～3.8%.The recovery rates of Moso bamboo extraction were 84.4%and 88.3%,respectively.This method can be used to measure the azoxystrobin content in treated materials,with simple and fast pretreatment steps,achieving a high precision of analysis results and the good reproducibility.关键词
嘧菌酯/超声波提取/高效液相色谱/防腐材Key words
azoxystrobin/ultrasonic extraction/high performance liquid chromatography(HPLC)/preservative-treated material分类
林学引用本文复制引用
张景朋,邵闯,蒋明亮..高效液相色谱法测定防腐材中嘧菌酯含量的方法研究[J].木材科学与技术,2025,39(1):64-70,7.基金项目
国家自然科学基金资助项目"木质素负载IPBC纳米微球载药体系的构建及对木材防霉性能的增效机制"(32301525). （32301525）
