南京农业大学学报2025，Vol.48Issue(2)：348-359,12.DOI:10.7685/jnau.202403018
细胞学结合转录组分析发掘绿豆花瓣开放及衰老关键基因
Identification of critical genes for petal opening and senescence in mungbean by cytology and transcriptome analysis
摘要
Abstract
[Objectives]This study was conducted to analyze the cytological changes in the process of mungbean flower blooming,explore the key genes with transcriptomic,and reveal the underlying molecular mechanism.[Methods]'Sulyu 1'was used in this study,cytological observations were conducted on the petals of each stage during the blooming process.Based on the cytological results,the critical stages of petal senescence were identified,and transcriptome sequencing was performed on the petals before and after these stages.Key genes were identified based on changes in expression levels and functional annotation,and their functions were ultimately verified through genetic transformation experiments in tabacco and Arabidopsis.[Results]Cytological experiments showed that from B2 to B4 stage,the epidermal cells of petals expanded,with cell wall thinning and chloroplasts degradation,subsequently enter programmed cell death rapidly,resulting in cell structure collapse.The transcriptome analysis detected cell wall synthesis and metabolism,as well as cell wall relaxation and cell expansion related genes,and identified 10 NAC family genes with significant changes in the expression levels,qPCR detected that VrNAC72b was a gene specifically expressed in petals,and its expression level rapidly increased at B3 and B4 stages.Expression of VrNAC72a/b in tobacco leaded to leaf yellowing,while overexpression of VrNAC72b in Arabidopsis leaded to early leaf senescence.NBT and DAB staining showed an increase in reactive oxygen species content in various tissues.[Conclusions]During the flowering process of mungbean,the cells expanded and died rapidly,leading to petal opening and subsequent littering.A large number of cell wall related genes were involved in this process,and the NAC family genes also played an important role.Among them,the expression level of VrNAC72b changed significantly,causing programmed cell death in petal cells.关键词
绿豆/花瓣/转录组/衰老/细胞学Key words
mungbean/petal/transcriptome/senescence/cytology分类
农业科技引用本文复制引用
林云,周琰琰,刘金洋,闫强,陈景斌,吴然然,薛晨晨,陈新,袁星星..细胞学结合转录组分析发掘绿豆花瓣开放及衰老关键基因[J].南京农业大学学报,2025,48(2):348-359,12.基金项目
国家自然科学基金项目(32301894) （32301894）
国家食用豆产业技术体系生物防治与综合防控岗位科学家项目(CARS-08-G15) （CARS-08-G15）
江苏省种业"揭榜挂帅"项目(JBGS[2021]004) （JBGS[2021]004）
江苏省"一带一路"国际合作项目(BZ2022005) （BZ2022005）
