肿瘤预防与治疗2025，Vol.38Issue(3)：171-181,11.DOI:10.3969/j.issn.1674-0904.2025.03.001
基于生物信息学和体外实验探讨蝙蝠葛碱治疗肺腺癌的靶点及机制
Investigating the Targets and Mechanisms of Dauricine in the Treatment of Lung Adenocarcinoma through Bioinformatics and In Vitro Experi-ments
摘要
Abstract
Objective:To explore dauricine(DAU)'s therapeutic effects and mechanisms in lung adenocarcinoma(LUAD)through bioinformatics and in vitro studies.Meth-ods:Potential targets of DAU were predicted using the SwissTargetPrediction database,while LUAD-related targets were ob-tained from The Cancer Genome Atlas database for differential expression analysis.Overlapping genes between DAU and LU-AD differentially expressed genes(DEGs)were identified and used to construct a protein-protein interaction network.Func-tional enrichment analysis of the overlapping genes was conducted using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses through the DAVID database,while patient survival analysis was performed via Sanger-box.A549 and NCI-H1975 cells were cultured in vitro,and the effects of different concentrations of DAU on cell prolifera-tion were assessed using the MTS assay.RT-qPCR was performed to analyze the effects of DAU on the mRNA expression lev-els of predicted target genes.Results:A total of 270 DAU-related targets were identified,and KEGG pathway analysis dem-onstrated significant enrichment of these targets in lung cancer-related pathways.A total of 5,367 DEGs were identified in LUAD,with 23 intersecting genes shared between DAU and LU AD.KEGG functional enrichment analysis revealed that the target genes were predominantly enriched in biological processes and pathways,including cell proliferation,neuroactive lig-and-receptor interactions,cancer,dopaminergic synapses,5-hydroxytryptaminergic synapses,and others.Survival analysis revealed that the genes MELK,HTR3A,AVPR1B,ADRB2,SLC6A3,SELP,ACHE,MTNR1A,RET,and OPRD1 are asso-ciated with the survival rate of LUAD patients.The results of the in vitro experiment demonstrated that DAU significantly in-hibited the proliferation of LUAD cells.DAU upregulates the mRNA expression levels of ADRA1A,ACHE,SLC6A4,CFD,TERT,and EZH2,while downregulating the mRNA expression levels of MELK,AURKA,and BCHE.Conclusion:This study demonstrated that DAU inhibits the proliferation of LUAD cells.By integrating bioinformatics analysis with in vitro ex-periments,the potential targets and mechanisms of DAU in LUAD treatment were validated,providing evidence to support its application in LUAD therapy.关键词
蝙蝠葛碱/肺腺癌/生物信息学Key words
Dauricine/Lung adenocarcinoma/Bioinformatics分类
医药卫生引用本文复制引用
程龙,熊媛,杨慧,钱铭,葛卫红..基于生物信息学和体外实验探讨蝙蝠葛碱治疗肺腺癌的靶点及机制[J].肿瘤预防与治疗,2025,38(3):171-181,11.基金项目
国家自然科学基金项目(编号:82204720) This study was supported by National Natural Science Foundation of China(No.82204720). （编号:82204720）
