中国生态农业学报（中英文）2025，Vol.33Issue(3)：531-540,10.DOI:10.12357/cjea.20240343
河北省典型湿地沉积物中抗生素抗性基因分布特征及其影响因素
Distribution patterns and influencing factors of antibiotic resistance genes in sediments from representative wetlands in Hebei Province
摘要
Abstract
Increasing research is currently focused on the ecological safety and health risks associated with the dissemination of anti-biotic resistance genes(ARGs)in the environment.Wetland ecosystems possess significant ecological,social,and economic value as crucial ecological barriers that effectively separate anthropogenic activities from the natural environment.The propagation of ARGs in wetland ecosystems poses a significant threat to both ecosystem integrity and human well-being,and has thus garnered increasing attention.In this study,we analyzed the distribution characteristics of ARGs and their influencing factors in representative coastal wetlands(Caofeidian and Nandagang)as well as inland freshwater wetlands(Baiyangdian and Hengshui lakes)located in Hebei Province using quantitative PCR and high-throughput sequencing techniques.The presence and abundance of ARGs,including two sulphonamide resistance genes(sul1 and sul2),eight tetracycline resistance genes(tetA,tetC,tetG,tetL,tetM,tetQ,tetW,and tetX),and one integrase gene(intI1),were detected and quantified in wetland sediments.The average total absolute abundance of ARGs de-tected in sediments in Nandagang(4.14×108 copies·g-1 in dry soil)was significantly higher than that in Caofeidian(1.21×108 copies·g-1 in dry soil),Baiyangdian Lake(1.11×108 copies·g-1 in dry soil)and Hengshui Lake(1.97×108 copies·g-1 in dry soil)(not in-cluding sampling site 1 in Hengshui Lake)(P<0.05).The absolute abundance of tetracycline resistance genes ranged from 8.91×103 to 8.42×107 copies·g-1 in dry soil,while the absolute abundance of sulfonamide resistance genes ranged from 9.63×105 to 2.62×107 copies·g-1 in dry soil).Notably,the distribution of ARGs in sediments varied even within the same lake.The absolute abundance of intI1 gene ranged from 3.26×105 to 1.11×108 copies·g-1 in dry soil,and a significant positive correlation was observed between the intI1 gene with tetA,tetL,sul1 and sul2 genes(P<0.05 or P<0.01 or P<0.001).These findings suggest that intI1 plays a crucial role in facilitating the dissemination of sediment ARGs.In addition,high-throughput sequencing revealed that Proteobacteria,Chloroflexi,Bacteroidota,Actinobacteriota,Acidobacteriota,Firmicutes,Desulfobacterota,Gemmatimonadota,Planctomycetota,Verrucomicrobi-ota,Nitrospirota,and Myxococcota were the predominant bacterial phyla in the sediment samples.These phyla collectively accoun-ted for 89.57%-96.05%of the total sediment bacterial community composition.Lower relative abundances of Proteobacteria and Firmicutes were observed in the coastal wetlands than that in the inland freshwater wetlands(P<0.05),whereas Planctomycetota ex-hibited the opposite trend.The Shannon and Simpson indices indicated a significantly higher alpha diversity of sediment bacterial communities in Caofeidian and Nandagang compared to Hengshui Lake.However,the non-metric multidimensional scaling analysis showed no significant differences in beta diversity among the Caofeidian,Nandagang,Baiyangdian Lake and Hengshui Lake sedi-ments.Variance partitioning analysis demonstrated that sediment physicochemical properties and bacterial community explained 60.78%of the ARGs distribution variance,with the bacterial community accounting for 37.42%,indicating its dominant role as a driving factor.The distribution of the bacterial community was also significantly influenced by the sediment physicochemical proper-ties,including SOM,TN,and TP.The findings of our study offer valuable data and scientific insights for the assessment of ARGs pollution in typical wetland sediments as well as for the preservation of wetland ecosystem security.关键词
湿地/沉积物/抗生素抗性基因/intI1基因/细菌群落结构Key words
wetland/sediment/antibiotic resistance genes/intI1 gene/bacterial community分类
资源环境引用本文复制引用
刘韵超,胡亚宁,龚玉敏,郑子英,丁林,李银辉,刘瑾,王凤花..河北省典型湿地沉积物中抗生素抗性基因分布特征及其影响因素[J].中国生态农业学报（中英文）,2025,33(3):531-540,10.基金项目
国家自然科学基金项目(42077358)、河北省自然科学基金项目(D2020205021)和河北师范大学科学基金项目(L2022B20)资助 This study was supported by the National Natural Science Foundation of China(42077358),the Natural Science Foundation of Hebei Province(D2020205021)and the Science Foundation of Hebei Normal University(L2022B20). （42077358）
