山西大学学报（自然科学版）2025，Vol.48Issue(2)：216-225,10.DOI:10.13451/j.sxu.ns.2024144
基于指纹图谱与化学计量学的淡竹叶质量控制研究
Quality Control Research of Lophatherum gracile Brongn.Based on Fingerprint and Chemometrics
摘要
Abstract
In this study,the fingerprint analysis and content determination of six indicator chemical components were performed on 15 batches of Lophatherum gracile Brongn.(LGB)by RP-HPLC method.The cluster analysis and principal component analysis of the contents of six components of LGB were performed using Origin 2019 software and SIMCA 14.0 software,respectively.The peak areas of the common peaks were analyzed for differential marker compounds according to orthogonal partial least squares-dis-criminant analysis(OPLS-DA).The results showed that 14 common peaks were matched on the fingerprints of LGB,and the similari-ty of the fingerprint was＞0.90.The content ranges of each component are expressed in mg·g-1 and are as follows:0.23～1.09,0.17～0.86,0.08～0.67,0.22～0.61,0.02～0.13,0.02～0.06,for isoorientin(Peak 8),orientin(Peak 9),swertiajaponin(Peak 10),vitexin(Peak 12),isovitexin(Peak 13),and luteoloside(Peak 14),respectively.The 15 batches of LGB samples were divided into 3 classes by cluster analysis,and the results of PCA were consistent with the results of cluster analysis.Finally,the five chemical components(orientin,swertiajaponin,vitexin,isovitexin,luteoloside)were screened as candidate differential marker compounds,which could be used as indicator components for quality control and comprehensive evaluation of LGB.关键词
HPLC指纹图谱/淡竹叶/化学计量学/黄酮类成分/质量评价Key words
HPLC fingerprint/Lophatherum gracile Brongn./chemometrics/flavonoids/quality assessment分类
药学引用本文复制引用
向欢,涂慕鑫,李玉,孟亦浩,蔚慧欣,王庆亮,张鑫,田俊生,秦雪梅..基于指纹图谱与化学计量学的淡竹叶质量控制研究[J].山西大学学报（自然科学版）,2025,48(2):216-225,10.基金项目
地产中药功效物质研究与利用山西省重点实验室项目(202105D121009) （202105D121009）
中国露酒植物提取与健康山西省重点实验室开放课题(202204010931030) （202204010931030）
