山西大学学报（自然科学版）2025，Vol.48Issue(2)：226-237,12.DOI:10.13451/j.sxu.ns.2024164
基于网络药理学和实验验证探讨黄芪耐缺氧的作用机制
Hypoxia Tolerance Effect and Mechanism of Astragali Radix Based on Network Pharmacology and Experimental Verification
摘要
Abstract
Network pharmacology was used to explore the potential mechanism underlying the hypoxia tolerance of Astragali Radix(AR).The primary active components and molecular targets of AR were identified using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database and a large number of literatures.Targets related to hypoxia toler-ance were screened through Online Mendelian Inheritance in Man(OMIM),GeneCards,DrugBank and Uniprot databases.The func-tional protein association(STRING)network was employed to screen key targets for protein-protein interaction(PPI)analysis.The biological processes and pathways involved were analyzed using Metascape database.Molecular docking and animal experiments were used for verification.The results showed that AR contains 24 main active ingredients and 238 action targets,comparing with 1 013 hypoxia tolerance targets identified by integrating multiple databases,resulting in 137 shared targets.Gene Ontology(GO)functional enrichment analysis revealed 1 916 biological processes(BP),102 cell compositions(CC),176 molecular functions(MF),and 199 KEGG pathways.Key targets for hypoxia tolerance in AR were identified as VEGFA,HIF-1α and IL-6.The potential active ingredients of AR are flavonoids and saponins such as quercetin and astragaloside IV.Molecular docking proved that quercetin,kaempferol,astragalosideⅠ,Ⅱ,Ⅲ and astragaloside IV demonstrated strong combination between VEGFA and HIF-1α.ELISA con-firmed the ability of AR to mitigate hypoxia suppressing VEGFA expression,a core target gene.The experiments of Drosophila fur-ther showed that AR has hypoxia tolerance.The study provides initial evidence that flavonoids and saponins in AR,such as astraga-loside IV and quercetin,can alleviate hypoxia by modulating key targets HIF-1α and VEGFA,offering potential implications for the utilization of Astragalus root in challenging environmental conditions such as high-altitude hypoxia.关键词
网络药理学/分子对接/黄芪/耐缺氧/血管内皮生长因子AKey words
network pharmacology/molecular docking/Astragali Radix/hypoxia tolerance/VEGFA分类
中医学引用本文复制引用
李爱平,张星星,朱云峰,但春凤,李犇,秦雪梅..基于网络药理学和实验验证探讨黄芪耐缺氧的作用机制[J].山西大学学报（自然科学版）,2025,48(2):226-237,12.基金项目
国家自然科学基金(82204595) （82204595）
山西省基础研究计划资助项目(202303021221070) （202303021221070）
博士后基金(340903) （340903）
山西省重点实验室(202105D121009) （202105D121009）
