山西大学学报（自然科学版）2025，Vol.48Issue(2)：252-264,13.DOI:10.13451/j.sxu.ns.2024171
网络药理学研究黄芪注射液次生成分治疗白细胞减少症的机制
Exploring the Mechanism of Secondary Components of Huangqi Injection in the Treatment of Leukopenia Using Network Pharmacology
摘要
Abstract
The objective of this study was to investigate the active components in secondary components of Huangqi injection and its mechanism for the effect on leukopenia using network pharmacology,molecular docking and in vivo experiments.According to the mass spectrometry information,the cleavage law of the reference substance and the reference literatures,the chemical compositions in secondary components of Huangqi injection were identified;Swiss Target Prediction platform was used to obtain the relevant tar-gets of the druggable components;leukopenia-related targets was screened by Gene cards and Disgenet databases;the intersections between the obtained component targets and disease-related targets were used to obtain the potential targets of secondary compo-nents of Huangqi injection in treating leukopenia,and the"component targets-disease targets"network was constructed by Cyto-scape 3.9.1 software to analyze the active ingredients;the common targets were imported into the String website for protein-protein interaction analysis to identify key targets;Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway were carried out through bioinformatics website;molecular docking and leukopenia mouse models were used to verify the results of network pharmacology.A total of 25 compounds were identified in secondary components of Huangqi injection,including 21 flavonoids and 4 saponins.There are 121 potential targets for secondary components of Huangqi injection in treating leukopenia,involving 486 biological processes,68 cellular components,96 molecular functions and 155 signaling pathways.Five potential ac-tive ingredients including Isorhamnetin-3-O-β-gentiobioside,Astragalin,6"-O-acetylCalycosin-7-O-D-glycoside,6"-O-acetylono-nin,and Wogonin,8 key target proteins including TP53,AKT1,IL6 and signaling pathways such as Phosphatidylinositol-3-kinase/protein kinase B(PI3K-Akt)signaling pathway,Janus kinase-signal transducer and activator of transcription(JAK-STAT)signaling pathway were obtained.The 5 potential active ingredients have strong binding affinity to the key targets.Secondary components of Huangqi injection could significantly regulate the contents of IL-6 and G-CSF in serum of leukopenia mice.Isorhamnetin-3-O-β-gentiobioside,Astragalin,6"-O-acetylCalycosin-7-O-D-glycoside,6"-O-acetylononin,and Wogonin are the active components of secondary components of Huangqi injection,which may act on key targets such as TP53,AKT1,IL6,etc,key pathways including PI3K-Akt signaling pathway,and JAK-STAT signaling pathway to play its therapeutic leukopenia.关键词
黄芪注射液次生成分/网络药理学/分子对接/体内实验/活性成分Key words
secondary components of Huangqi injection/network pharmacology/molecular docking/in vivo/active components分类
中医学引用本文复制引用
曲婷丽,张楠,周小雪..网络药理学研究黄芪注射液次生成分治疗白细胞减少症的机制[J].山西大学学报（自然科学版）,2025,48(2):252-264,13.基金项目
山西省科技厅基础研究项目(20210302124586) （20210302124586）
山西省重点研发项目(202102130501023) （202102130501023）
太原市科技局"双百攻关行动"首批关键核心技术攻关"揭榜挂帅"项目(2024TYJB0150) （2024TYJB0150）
山西省技术创新中心项目(202104010911006) （202104010911006）
山西省教育厅教改项目(J20230518) （J20230518）
