现代检验医学杂志2025，Vol.40Issue(2)：1-5,5.DOI:10.3969/j.issn.1671-7414.2025.02.001
下调ACTG1抑制PI3K/Akt信号通路相关蛋白表达促进胃癌细胞HGC-27凋亡机制研究
Mechanism of ACTG1 Downregulation Inhibits the Expression of Protein Associated to PI3K/Akt Signaling Pathway and Promotes Gastric Cancer Cells Line HGC-27 Apoptosis
摘要
Abstract
Objective To investigate the effect and mechanism of actin gamma1(ACTG1)downregulation on apoptosis in gastric cancer cells line HGC-27.Methods Stable ACTG1 knockdown HGC-27 cell line was constructed by CRISPR/Cas9,ACTG1 knockdown was verified by DNA sequencing and Western blot,apoptosis rate was detected by flow cytometry,Western blot detected the expression level of apoptosis-related proteins Bcl2-associated X protein(Bax),B cell lymphoma 2 family protein(Bcl2)and PI3K/AKT signal pathway-related proteins AKT and p-AKT.Results HGC-27 cell lines with stable knockdown of ACTG1 were constructed,and the ACTG1 protein levels were decreased in the sgACTG1-1 and sgACTG1-2 groups compared with the Control group.Compared with the apoptosis rate in the Control group(6.54%±0.67%),cell apoptosis rate in the sgACTG1-1 and sgACTG1-2 groups(10.11%±0.46%,14.67%±0.17%)were significantly increased,the differences were statistically significant(t=-7.58,-20.28,all P<0.01).Compared the Control group,the Bax protein levels in the sgACTG1-1 and sgACTG1-2 groups(0.89±0.02,1.00±0.08 vs 0.71±0.03)were significantly increased(t=-8.14,-5.87),the level of Bcl2 protein(0.49±0.06,0.39±0.06 vs 0.65±0.07)were significantly decreased(t=3.09,5.35),the differences were statistically significant(all P<0.05).Compared with the Control group,the AKT protein levels in the sgACTG1-1 and sgACTG1-2 groups(0.95±0.10,0.43±0.09 vs 1.17±0.06)and the P-AKT protein level(0.38±0.08,0.28±0.12 vs 0.70±0.14)were significantly decreased,the differences were statistically significant(t=3.20,12.13;3.44,3.85,all P<0.05).Conclusion Downregulation of ACTG1 inhibits the expression of PI3K/Akt signaling pathway related proteins AKT,p-AKT and promotes gastric cancer cells line HGC-27 apoptosis.关键词
γ-肌动蛋白/人胃癌细胞(未分化)/磷脂酰肌醇3-激酶/蛋白激酶B/凋亡Key words
ACTG1/HGC-27/PI3K/Akt/apoptosis分类
临床医学引用本文复制引用
李寿敏,兰冰雪,潘莉,孙铭,崔淼淼,韦四喜,黄海..下调ACTG1抑制PI3K/Akt信号通路相关蛋白表达促进胃癌细胞HGC-27凋亡机制研究[J].现代检验医学杂志,2025,40(2):1-5,5.基金项目
国家自然科学基金(82060442) （82060442）
2021年贵州医科大学附属医院博士科研启动基金(gyfybsky-2021-39) （gyfybsky-2021-39）
贵州医科大学国家自然科学基金培育项目(21NSFCP05) （21NSFCP05）
2021年贵州医科大学附属医院国家自然科学基金培育项目(gyfynsfc-2021-11). （gyfynsfc-2021-11）
