摘要
Abstract
Objective To explore the expression of long non-coding RNA hypoxia-inducible factor 1 alpha antisense RNA-1(lncRNA HIF1A-AS1)levels in placenta and cord blood of pregnant women with preeclampsia(PE)and its clinical significance.Methods Fifty pregnant women with PE who underwent cesarean section in Renji Hospital,Shanghai Jiaotong University School of Medicine from January 2020 to January 2022 were selected as the research subjects,including 24 pregnant women with mild PE and 26 pregnant women with severe PE.During the same period,25 healthy pregnant women who underwent cesarean section at the hospital were selected as the control group.The general data on the admission of all patients were collected.The real-time fluorescence quantitative qRT-PCR method was used to detect the expression level of lncRNA HIF1A-AS1 in pregnant women's placenta tissue and cord blood.The relationship between lncRNA HIF1A-AS1 level and pregnancy outcome was analyzed.The Pearson method was used to analyze the correlation between the relative expression level of lncRNA HIF1A-AS1 mRNA in placenta tissue and the level of lncRNA HIF1A-AS1 in cord blood.The receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of lncRNA HIF1A-AS1 in placenta and cord blood for severe PE.Logistic regression was used to analyze the influencing factors of severe PE.Results Compared with the control group,the level of lncRNA HIF1A-AS1(2.76±0.36,1.83±0.29 vs 1.03±0.21)was elevated in the placental tissues of pregnant women with PE in the severe and mild groups(q=29.687,13.456),and the expression level of lncRNA HIF1A-AS1 in the severe group was higher than that in the mild group(q=15.792),and the differences were statistically significant(all P＜0.05).Compared with the control group,the level of lncRNA HIF1A-AS1(4.68±0.58,2.66±0.49 vs 1.06±0.34)was elevated in the cord blood of pregnant women with PE in the severe and mild groups(q=37.942,14.437),and the expression level of lncRNA HIF1A-AS1 in the severe group was higher than that in the mild group(q=20.951),and the differences were statistically significant(all P<0.05).The incidence of adverse pregnancy outcomes in placenta and cord blood lncRNA HIF1A-AS1 high expression group was significantly higher than that in lncRNA HIF1A-AS1 low expression group(χ2=7.714,5.773,all P<0.05).Pearson correlation analysis showed that the expression level of lncRNA HIF1A-AS1 mRNA in placenta tissue was positively correlated with the level of lncRNA HIF1A-AS1 in cord blood(r=0.546,P<0.05).ROC curve analysis showed that the area under the curve of lncRNA HIF1A-AS1 in the placenta for the diagnosis of severe PE was 0.788,with a sensitivity and a specificity of 80.80%and 80.00%,respectively.The AUC of lncRNA HIF1A-AS1 in the cord blood for the diagnosis of severe PE was 0.829,with a sensitivity and a specificity of 80.80%and 88.00%,respectively.Logistic regression analysis showed that 24-hour proteinuria,and placental and cord blood lncRNA HIF1A-AS1 levels were independent risk factors for severe PE(all P<0.05).Conclusion LncRNA HIF1A-AS1 in the placenta and cord blood of PE pregnant women is significantly up-regulated,and it increases with the aggravation of the disease.Placenta and cord blood lncRNA HIF1A-AS1 have a certain diagnostic value for the occurrence of severe PE.Detection of lncRNA HIF1A-AS1 has important significance for the early and accurate diagnosis of PE and the prediction of the disease.关键词
子痫前期/长链非编码RNA低氧诱导因子1α反义RNA-1/胎盘/脐血Key words
pre-eclampsia/lncRNA HIF1A-AS1/placenta/cord blood分类
医药卫生
