沈阳医学院学报2025，Vol.27Issue(2)：149-154,186,7.DOI:10.16753/j.cnki.1008-2344.2025.02.007
电针刺激上调大脑中动脉栓塞大鼠海马CA1区Nrf2、GPX4的表达
Electroacupuncture stimulation up-regulates the expression of Nrf2 and GPX4 in the hippocampal CA1 region of rats with middle cerebral artery occlusion
摘要
Abstract
Objective:To investigate the effects of electroacupuncture stimulation on the expression of Nrf2 and GPX4 proteins in the hippocampal CA1 region of rats with middle cerebral artery occlusion(MCAO)and its neuroprotective mechanism.Methods:Forty-eight rats were divided into the normal group(Normal),the sham operation control group(Sham),the model group(MCAO)and the electroacupuncture group(EA)according to the random number table method.The right middle cerebral artery occlusion model was established in the MCAO and EA groups by the modified thread embolization method.Starting on day 1 post-modeling,the EA group received electroacupuncture stimulation at the"Baihui"point(GV20)and left"Neiguan"point(PC6)for 30 min daily over 14 d.The Normal group received no treatment,and the Sham group only underwent vascular isolation without inserting the suture.Neurological function of rats in each group were scored before and after modeling and before and after electroacupuncture stimulation using the Zea Longa scoring scale.The structure and arrangement of Nissl-positive cells in the hippocampal CA1 region were observed by Nissl staining.Western blot and immunohistochemical staining were used to determine the expression levels of Nrf2 and GPX4 proteins in the hippocampal CA1 region.Results:Compared with the Normal group,the Sham group showed no neurological deficits,abundant Nissl-positive cells,and no statistically significant differences in Nrf2 and GPX4 protein expression or positive cell counts.Compared with the Sham group,the MCAO group exhibited elevated Zea Longa score,reduced Nissl-positive cell counts,and decreased Nrf2 and GPX4 protein expression and positive cell counts(P＜0.05).In contrast,the EA group demonstrated lower Zea Longa scores,increased Nissl-positive cell counts,and elevated Nrf2 and GPX4 protein expression and positive cell counts compared to the MCAO group(P＜0.05).Conclusion:Electroacupuncture stimulation may protect against neuronal damage in the hippocampal CA1 region of MCAO rats,potentially through up-regulation of Nrf2 and GPX4 expression.关键词
电针/缺血性卒中/海马CA1区/Nrf2/GPX4Key words
electroacupuncture/ischemic stroke/hippocampal CA1/Nrf2/GPX4分类
临床医学引用本文复制引用
吴琪,金毅豪,刘星辰,吴锋,缪化春,李怀斌..电针刺激上调大脑中动脉栓塞大鼠海马CA1区Nrf2、GPX4的表达[J].沈阳医学院学报,2025,27(2):149-154,186,7.基金项目
安徽省高等学校省级质量工程项目(No.2019jxtd072) （No.2019jxtd072）
安徽省高等学校科研计划重点项目(No.2024AH051927) （No.2024AH051927）
皖南医学院大学生科研基金研究生类项目(No.WK2023XS56) （No.WK2023XS56）
