时珍国医国药2025，Vol.36Issue(4)：609-615,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-0402
基于上皮-间质转化探讨哮喘平冲剂干预支气管哮喘气道重塑的作用机制
Investigating the mechanism of Xiaochuanping Granule in intervening airway remodeling in bronchial asthma based on epithelial-mesenchymal transition
摘要
Abstract
Objective To investigate the mechanism by which Xiaochuanping Granule(XCPG)inhibits the airway remodeling in asthma model rats by regulating epithelial-mesenchymal transition(EMT).Methods Seventy male Sprague-Dawley(SD)rats were randomly divided into the blank control group,model group,XCPG low-dose,medium-dose,and high-dose groups(3.87g·kg-1、7.74g·kg-1、15.48g·kg-1),Suhuang group(0.43g·kg-1),with 10 rats in each group.Except for the blank control group,all the other groups were sensitized and then injected with ovalbumin(OVA)to create asthma in the rat models.Each rat group was given particular medications through a tube for 21 days in a row,and their general condition was observed.The pathological changes in the rat lung tis-sues and the deposition of airway collagen were observed using hematoxylin-eosin(HE)and Masson staining.Enzyme-linked immu-nosorbent assay(ELISA)was used to check the levels of IL-17,IL-22,and IgE in the blood of rats.Real-time fluorescent quantita-tive polymerase chain reaction(RT-qPCR)was used to check the mRNA expression levels of transforming growth factor Beta 1(TGF-β1),Smad3,and Smad7 in the lung tissues.Western blot(WB)was used to detect the expression levels of TGF-β1,Smad3,Smad7,alpha-smooth muscle actin(α-SMA),E-cadherin,and Vimentin in the lung tissues.Results Compared to the blank con-trol group,HE and Masson staining in the rat model group showed that the bronchial and alveolar wall structure was disordered,the epi-thelial cells were deformed and necrotic,a large number of inflammatory cells were infiltrated,the deposition of airway collagen fibers was significantly increased,and the tube wall was thickened;serum IgE,IL-17,and IL-22 levels were significantly elevated(P<0.01);TGF-β1 mRNA,Smad3 mRNA,and TGF-β1,Smad3,a-SMA,and wave protein expressions in the lung tissues were significantly upregulated(P<0.01),while Smad7 mRNA,Smad7,and E-cadherin expressions were significantly downregulated(P<0.01).Compared with the model group,in the XCPG groups with different dosages,the Suhuang group,and the dexamethasone group,the de-gree of inflammatory cell infiltration and collagen fiber accumulation in the rat lung tissues were decreased,and the thickness of the tra-cheal wall was decreased;serum IgE,IL-17,and IL-22 levels significantly decreased(P<0.05);TGF-β1 mRNA,Smad3 mR-NA,TGF-β1,Smad3,a-SMA,and wave protein expression in the lung tissues were significantly downregulated(P<0.05),while Smad7 mRNA,Smad7,and E-cadherin expression were significantly upregulated(P<0.05).Conclusion XCPG can reduce the ex-pressions of TGF-β1 and Smad3 while upregulating Smad7 in the lung tissues,thereby inhibiting EMT and alleviating airway remodeling in asthma.关键词
哮喘平冲剂/支气管哮喘/上皮-间质转化/气道重塑/TGF-β1/Smad3/Smad7Key words
Xiaochuanping Granule/Bronchial asthma/Epithelial-mesenchymal transition/Airway remodeling/TGF-β1/Smad3/Smad7分类
医药卫生引用本文复制引用
史梦蓉,张念志,张芮,周静,孙小强,张倩倩,褚文丰,金纯纯..基于上皮-间质转化探讨哮喘平冲剂干预支气管哮喘气道重塑的作用机制[J].时珍国医国药,2025,36(4):609-615,7.基金项目
国家自然科学基金面上项目(82174312) （82174312）
国家中医药管理局高水平中医药重点学科建设项目(国中医药人教函[2023]85号) （国中医药人教函[2023]85号）
安徽省中医肺病临床医学研究中心项目(皖科社[2020]41号) （皖科社[2020]41号）
