时珍国医国药2025，Vol.36Issue(4)：616-621,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0403
黄连干预下雌激素水平对小鼠粪便菌群结构影响的研究
Exploring the effect of Rhizoma Coptidis in intervening the estrogen levels on the fecal microbi-ota structure of mice
摘要
Abstract
Objective To explore the effect of Rhizoma Coptidis in intervening the estrogen levels on the structure of fecal microbiota in mice,providing new theoretical evidence for the pharmacological effects and clinical applications of Rhizoma Coptidis.Methods Specific Pathogen Free(SPF)female mice were randomly divided into the sham operation group(SHAM),the ovariectomy group(OVX),and the ovariectomy+estrogen group(E2).After oral administration of the same dose of Rhizoma Coptidis,high-throughput sequencing technology was used to perform 16S rRNA gene sequencing on the fecal samples of the mice,observing changes in the fecal microbiota structure across the groups.Results Ovariectomy altered the estrogen levels in mice,resulting in significant changes in the fecal microbi-ota structure of the OVX group compared to the SHAM group(P<0.05).With estrogen treatment,the microbiota structure of the E2 group was similar to that of the SHAM group.Conclusion The effect of Rhizoma Coptidis in intervening the estrogen levels on the struc-ture of fecal microbiota in mice was significant.This study provides new insights for understanding the interaction between estrogen and gut microbiota,as well as for the use of Rhizoma Coptidis based on sex differences.关键词
雌激素水平/黄连/粪便菌群/结构Key words
Estrogen levels/Rhizoma Coptidis/Fecal microbiota/Structure分类
医药卫生引用本文复制引用
朱晨,段学清,田维毅..黄连干预下雌激素水平对小鼠粪便菌群结构影响的研究[J].时珍国医国药,2025,36(4):616-621,6.基金项目
国家自然科学基金项目(82060824) （82060824）
贵州省基础研究计划项目(黔科合基础-ZK[2022]一般512) （黔科合基础-ZK[2022]一般512）
贵州省基础研究计划项目(黔科合基础-ZK[2022]一般460) （黔科合基础-ZK[2022]一般460）
