时珍国医国药2025，Vol.36Issue(4)：622-627,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0404
基于自噬和凋亡探究补肾活血方改善不明原因复发性流产妊娠结局的机制研究
Mechanism Study of Bushen Huoxue Decoction in Improving Pregnancy Outcomes of Unex-plained Recurrent Spontaneous Abortion Based on Autophagy and Apoptosis
摘要
Abstract
Objective To investigate the intervention mechanism of Bushen Huoxue Decoction(BSHXD)on unexplained recurrent spontaneous abortion(URSA)from the perspective of autophagy and apoptosis based on animal experiments.Methods CBA/J female mice were caged with BALB/c male mice to establish a normal pregnancy model,which were then divided into the blank group and the 3-methyladenine(3-MA)group.DBA/2 male mice were caged with CBA/J females to establish the URSA pregnancy model,which were then divided into the model group and the BSHXD group,and the mice all groups were given drug for 8 consecutive days from the 1st day of the discovery of vaginal plugs.The mice were euthanized 2 hours after gavage or intraperitoneal injection on the 8th day of the female mice's gestation,embryo resorption was observed in each group,and the embryo resorption rate was calculated.Hematoxylin-e-osin(HE)staining was used to observe the histological changes of placenta and Western blot(WB)to detect the expression levels of LC3,Beclin1,P62,Caspase3,bax,and bcl-2 in the placental tissues of pregnant rats in each group.Results Compared with the blank group,the embryo absorption rate in the model group and the 3-MA group was significantly increased(P<0.05);compared with the model group,the embryo absorption rate in the BSHXD group was significantly decreased(P<0.05).HE staining results showed that the extent of cell necrosis in the placental tissues was reduced in the BSHXD group.WB results showed that compared with the blank group,the expression levels of LC3,Beclin1,and Bcl-2 proteins in the placental tissues of the model group and 3-MA group were sig-nificantly decreased,while the expression levels of P62,Caspase3,and Bax were significantly increased(P<0.05).Compared with the model group,the expression levels of LC3,Beclin1,and Bcl-2 proteins in the placental tissues of the BSHXD group were significantly increased,while the expression levels of P62,Caspase3,and Bax proteins were significantly decreased(P<0.05).Conclusion URSA pregnant mice exhibit reduced autophagy levels,increased apoptosis,and high embryo resorption rates.Decreased autophagy level may lead to increased apoptosis,resulting in increased embryo resorption rate.The improvement of URSA pregnancy outcomes by BSHXD may be related to its increase of autophagy level and decrease of apoptosis in placental tissue cells.关键词
不明原因复发性流产/补肾活血方/细胞自噬/细胞凋亡Key words
Unexplained recurrent spontaneous abortion/Bushen Huoxue Decoction/Autophagy/Apoptosis分类
医药卫生引用本文复制引用
任佳杰,高倩倩,牛丁忍,郭鲁秦,冯晓玲..基于自噬和凋亡探究补肾活血方改善不明原因复发性流产妊娠结局的机制研究[J].时珍国医国药,2025,36(4):622-627,6.基金项目
国家自然科学基金面上项目(82174421) （82174421）
黑龙江省自然科学基金项目(ZD2021H006) （ZD2021H006）
黑龙江省重点研发计划项目(2022ZX60C19) （2022ZX60C19）
黑龙江中医药大学研究生创新科研项目(2022yjscx003) （2022yjscx003）
