时珍国医国药2025，Vol.36Issue(4)：636-643,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-0406
基于JAK2/STAT3信号通路研究左归丸调控睾丸炎性微环境提高精子质量的作用机制
Investigating the mechanism of Zuogui Pill in regulating testis inflammatory microenvironment and improving sperm quality through the JAK2/STAT3 signaling pathway
摘要
Abstract
Objective To explore the mechanism of Zuogui Pill(ZGP)in regulating the testicular inflammatory microenvironment,im-proving the blood-testis barrier(BTB)permeability,and the enhancing sperm quality in rats with congenital kidney deficiency based on the JAK2/STAT3 signaling pathway.Methods A rat model of congenital kidney deficiency was established using the chronic unpredicta-ble mild stress(CUMS)method to simulate the fear stress during pregnancy.Behavioral experiments were conducted to evaluate the pregnant rat models.TUNEL staining was used to observe the apoptosis of testicular spermatogenic cells in the offspring models.Hema-toxylin-eosin staining(HE)was used to observe the pathological microstructure of the testicular tissues.An automatic sperm analyzer was employed to measure sperm density and motility.Western blot(WB)was used to detect the protein expressions of JAK2,STAT3 and Connexin43(Cx43)in the testicular tissues,and enzyme-linked immunosorbent assay(ELISA)was used to measure serum in-flammatory factors,including interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels.Results Compared with the blank group,the model group showed abnormal apoptosis of testicular spermatogenic cells,widespread inflammatory cell infiltration,and significant reductions in sperm density and motility.Serum levels of IL-6 and TNF-α,as well as JAK2 and STAT3 protein expressions in the testicular tissues,were significantly increased,while Cx43 expression was significantly decreased.In the prevention group,com-pared with the model group,the number of spermatogenic cells at all stages in the testis was increased,sperm density and motility were significantly higher,serum IL-6 and TNF-α levels,as well as STAT3 expression in testicular tissue,were significantly reduced,JAK2 expression in the testicular tissues was significantly decreased,and Cx43 expression was significantly elevated.Conclusion ZGP may im-prove the abnormal apoptosis of spermatogenic cells in adult rats by promoting the proliferation of supportive cell populations during em-bryonic development in rats,inhibiting the continuous activation of the JAK2/STAT3 signaling pathway by serum inflammatory cytokines IL-6 and TNF-α,enhancing the expression level of Cx43 protein,and restoring the permeability of BTB,ultimately improving sperm quality.关键词
睾丸炎性微环境/左归丸/先天肾虚/血睾屏障/精子质量Key words
Testis inflammatory microenvironment/Zuogui Pill/Congenital kidney deficiency/Blood-testis barrier/Sperm quality分类
医药卫生引用本文复制引用
任方楠,曹继刚,萧闵,王望,郝恒..基于JAK2/STAT3信号通路研究左归丸调控睾丸炎性微环境提高精子质量的作用机制[J].时珍国医国药,2025,36(4):636-643,8.基金项目
国家自然科学基金青年项目(82104704) （82104704）
