现代医药卫生2025，Vol.41Issue(3)：577-583,7.DOI:10.3969/j.issn.1009-5519.2025.03.001
肉苁蓉活性成分毛蕊花糖苷通过诱导自噬对NAFLD的作用研究
Study on the effect of Acteoside(an active ingredient in Cistanche deserticola)on NAFLD by inducing autophagy
摘要
Abstract
Objective To explore the autophagy inducing function of active ingredient Acteoside(ACT)in Cistanche deserticola and its effect on non-alcoholic fatty liver disease(NAFLD).Methods To take Echi-nacoside(ECH)and ACT as research objects,100 μmol/L ACT and ECH were combined with autophagy ly-sosome inhibitor bleomycin A1(BafA1)for intervention treatment.Protein immunoblotting was used to de-tect the relative expression level of autophagy marker protein microtubule associated protein 1 light chain 3(LC3-Ⅱ).HepG2 cells were intervened with different concentrations of ACT in vitro,and cell viability was detected using the tetrazolium salt method.In vitro intervention of HepG2 cells with different concentrations of ACT combined with BafA1,and detection of LC3-Ⅱ protein expression levels using protein immunoblotting technology.A NAFLD cell model constructed in vitro with drug intervention was used to stain lipid droplets using Oil Red O staining method and detect intracellular triglyceride(TG)levels.Observe the localization of LC3 protein and lipid droplets through immunofluorescence technology.Results ACT can significantly induce autophagy in HepG2 cells,and the expression level of LC3-Ⅱ protein of ACT+BafA1 group was significantly increased compared to ACT group(1.36±0.11,0.28±0.02,respectively),with statistical significance differ-ence(P<0.05).Different concentrations of ACT intervention had no effect on HepG2 cell viability after 24 and 48 hours,and the differences were not statistically significant(P>0.05).50 μmol/L ACT significantly induced autophagy in HepG2 cells,and the expression level of LC3-Ⅱ protein was significantly increased(1.83±0.53,0.35±0.18,respectively).The TG level of HepG2 cells in the ACT intervention group[(19.11±1.68)μg/mg]was significantly lower than that in the model group[(34.15±1.90)μg/mg],and the differences were statistically significant(P<0.05).The number of positive lipid droplets in HepG2 cells in the ACT intervention group decreased significantly,and the number of autophagosomes inside the cells in-creased significantly,showing clear co localization with lipid droplets in space.Conclusion Cistanche deserti-cola ACT has significant autophagy induction ability,and it may have potential preventive and therapeutic effects on NAFLD.关键词
非酒精性脂肪肝/肉苁蓉/毛蕊花糖苷/自噬Key words
Non-alcoholic fatty liver disease/Cistanche deserticola/Acteoside/Autophagy分类
中医学引用本文复制引用
伊力亚斯·艾萨,木塔力甫·艾买提,任丽君,付瑾..肉苁蓉活性成分毛蕊花糖苷通过诱导自噬对NAFLD的作用研究[J].现代医药卫生,2025,41(3):577-583,7.基金项目
新疆维吾尔自治区自然科学基金项目(2022D01B41). （2022D01B41）
