摘要
Abstract
Objective To investigate the expression levels of long non-coding RNA(LncRNA)FGD5-AS1 and microRNA(miR)-497-5p in serum of patients with intracerebral hemorrhage(ICH),and their correlation with neurological impairment and prognosis.Methods A total of 103 ICH patients diagnosed at Jianli People's Hospital of Hubei Province from June 2021 to March 2023 were collected as the ICH group,and 127 healthy individuals were selected as the control group.ICH patients were categorized into mild(26 cases),moderate(43 cases),and severe(34 cases)groups according to the National Institutes of Health Stroke Scale(NIHSS)scores.After a 3-month follow-up,patients were divided into a good prognosis group(59 cases)and a poor prognosis group(44 cases)according to the Glasgow Outcome Scale(GOS)scores.RT-qPCR was applied to analyze the levels of serum LncRNA FGD5-AS1 and miR-497-5p.Logistic regression was applied to analyze the influencing factors of poor prognosis in ICH patients.Receiver operating characteristic(ROC)curve was plotted to analyze the diagnostic value of serum LncRNA FGD5-AS1 and miR-497-5p levels for poor prognosis.Results Compared with the control group,the level of LncRNA FGD5-AS1 in the ICH group was reduced(t=10.429,P<0.05),while the level of miR-497-5p was increased(t=-30.947,P<0.05).The LncRNA FGD5-AS1 and miR-497-5p levels were correlated with the disease severity assessed by NIHSS scores in ICH patients(r=-0.615,0.673;P<0.05).Compared with the good prognosis group,the LncRNA FGD5-AS1 level in the poor prognosis group was reduced,and the miR-497-5p level was increased(t=7.944,-7.420;P<0.05).The area under the curve(AUC)of the combined diagnosis of LncRNA FGD5-AS1 and miR-497-5p for poor prognosis of ICH was 0.924(95%CI=0.870-0.978),with a sensitivity of 93.18%and a specificity of 83.05%.LncRNA FGD5-AS1 was a protective factor for poor prognosis in ICH patients(OR=0.694,P<0.05),while NIHSS score,hematoma volume,and miR-497-5p were risk factors for poor prognosis in ICH patients(OR=2.758,2.542,2.209;P<0.05).Conclusion The serum LncRNA FGD5-AS1 expression is low and miR-497-5p expression is high in ICH patients,and both are closely related to neurological impairment and poor prognosis.关键词
脑出血/长链非编码RNA FGD5-AS1/微小RNA-497-5p/神经功能损伤/预后Key words
Intracerebral hemorrhage/Long non-coding RNA FGD5-AS1/MicroRNA-497-5p/Neurological impairment/Prognosis
