园艺学报2025，Vol.52Issue(2)：292-308,17.DOI:10.16420/j.issn.0513-353x.2024-0304
菠萝AcKNOX1影响叶缘发育的功能研究
Functional Studies of Pineapple AcKNOX1 on Leaf Margin Development
摘要
Abstract
In this study,AcKNOX1 was cloned from pineapple'Tainong16',which is a homeobox gene,belongs to the three amino acid loop extension superfamily.The gene's open reading frame was 990 bp with 329 amino acids.The protein was localized in the nucleus.In addition to the core promoter elements TATA-box and CAAT-box,the AcKNOX1 promoter region contained many elements related to light,hormonal and abiotic stress responses.AcKNOX1 was expressed in different tissues with the highest expression in the stem,followed by leaves,which suggested a certain tissue specificity.AcKNOX1 showed higher expression in spiny leaf materials than in spineless leaf materials.The expression of AcKNOX1 was up-regulated by short-term GA3,ABA,and 6-BA stress.Ectopic overexpression of AcKNOX1 in Arabidopsis thaliana suggested that transformants showed larger and deeper serrations.Analysis of interaction proteins obtained by Yeast one and two hybrid system showed that AcKNOXl could interact and activate AcCUC1 and AcCUC2 promoter regions.And AcKNOX1 interacted with AcBLH1 and AcBLH4 to form a heterodimer.And the inteaction was verified by Luciferase complementation imaging assay(LCI)and Bimolecular fluorescence complementation(BIFC).The results revealed that AcKNOX1 gene played a vital role in the regulation of pineapple leaf margin development.关键词
菠萝/叶缘发育/AcKNOX1/蛋白互作Key words
Ananas comosus/leaf margin development/AcKNOX1/protein-interation分类
农业科技引用本文复制引用
荆永琳,陈浪欣,李俊国,王加宾,王小冰,杨庆全,孟春阳,徐立..菠萝AcKNOX1影响叶缘发育的功能研究[J].园艺学报,2025,52(2):292-308,17.基金项目
海南省自然科学基金项目(321QN322) （321QN322）
国家自然科学基金项目(31372106) （31372106）
