园艺学报2025，Vol.52Issue(2)：309-321,13.DOI:10.16420/j.issn.0513-353x.2023-0903
黄瓜CsCuAO家族基因鉴定及其在不定根形成中的作用研究
Identification of CsCuAO Gene Family in Cucumber and Their Regulatory Roles in Adventitious Root Formation
摘要
Abstract
Seven copper amine oxidase genes(CsCuAO)were identified from the genome of cucumber(Cucumis sativus L.)using bioinformatic techniques.The amino acid lengths of these deduced CsCuAO proteins ranged from 652 to 710 aa,with the isoelectric points(pi)ranging from 5.41 to 8.31.Phylogenetic analysis indicated that CsCuAOs were divided into three subfamily groups,and the genetic structure of the same subfamily had a high similarity.Treatment with putrescine,the CuAO enzymatic substrate,could promote adventitious root formation in cucumber hypocotyls,while application with aminoguanidine,a CuAO specific inhibitor,could inhibit adventitious root formation,with less adventitious root number and shorter adventitious root length.H2O2,but not the other CuAO's catalytic products,could reverse the effect of aminoguanidine on adventitious root formation.The results of qRT-PCR suggested that the expression of CsCuAOβ and CsCuAOζ were dominantly induced at the early stage of adventitious root formation(24 h),while CsCuAOγ1 was significantly induced at the late stage(72-120 h).Further analysis indicated that the expression of CsCuAOβ and CsCuAOζwas inhibited by IAA,but induced by ACC,the synthesis precursor of ethylene.Both IAA and ACC did not exert a significant influence on the expression of CsCuAOγ1.H2O2 could induce the expression of CsCuAOβ,CsCuAOζ and CsCuAOγ1 in different degrees.Transient overexpression of CsCuAOβ,CsCuAOζ and CsCuAOγ1 in cucumber cotyledons could increase the CuAO enzymatic activity and H2O2 contents,however,only CsCuAOβ overexpression could promote adventitious root formation at the base of the cotyledon petioles,while CsCuAOγ1 and CsCuAOζ overexpression had no significant effect on adventitious root formation.The results showed that CsCuAOβ gene responds to ethylene signal,and generates H2O2 and thereby promotes adventitious root formation in cucumber.关键词
黄瓜/铜胺氧化酶/不定根/过氧化氢/基因鉴定Key words
cucumber/copper amine oxidase/adventitious root/hydrogen peroxide/gene identification分类
农业科技引用本文复制引用
温正阳,孙靖博,张梦夏,张锋,董春娟..黄瓜CsCuAO家族基因鉴定及其在不定根形成中的作用研究[J].园艺学报,2025,52(2):309-321,13.基金项目
国家自然科学基金项目(32372795) （32372795）
现代农业产业技术体系建设专项资助(CARS-25) （CARS-25）
中国农业科学院科技创新工程项目(CAAS-ZDRW202302,CAAS-ASTIP-IVFCAAS) （CAAS-ZDRW202302,CAAS-ASTIP-IVFCAAS）
