园艺学报2025，Vol.52Issue(2)：322-336,15.DOI:10.16420/j.issn.0513-353x.2024-0620
辣椒疫病不同抗性品种内生微生物群落多样性、结构及功能分析
Analysis of Endogenous Microbial Community Diversity,Structure and Function of Pepper Different Resistant Phytophthora Blight Cultivars
摘要
Abstract
The total DNA of the roots,stems and leaves samples of peppers were extracted from the susceptible pepper cultivars(Fudijian and Qiemen)and resistant pepper cultivars(CM334 and SJ-0948)respectively,and the v3-v4 region of bacterial 16S rRNA gene and the fungal ITS1 region were sequenced using the Illumina platform.The results showed that there were significant differences in the composition of endophytic microorganisms in different pepper cultivars under natural planting.The diversity and richness index of fungal communities in roots,stems and leaves of susceptible cultivars.were significantly lower than those of resistant pepper cultivars.At the phylum level,the dominant bacterial and fungal phyla in the rhizosphere,root and leaves between two types of resistant peppers were the same,but the relative abundance of fungal Ascomycota phylum in the roots,stems and leaves of susceptible pepper(Fudijian)was much higher than that of resistant cultivars at the genus level,the dominant bacterial genera of the resistant pepper(CM334)were Mycobacterium(36.96％)in the root,and 2013Ark19i(15.21％),Photobacterium(5.50％),and Methylobacterium_Methylorubrum(4.11％)in stem,and Aureimonas(3.02％)in the leaves.Moreover,there were more harmful fungal genera in the endogenous parts of susceptible pepper(Fudijian),such as Fusarium accounting for 9.50％and 4.23％in roots and stems,and Cladosporium accounting for 45.46％and 51.74％in stems and leaves,respectively,which were significantly higher than those of two resistant cultivars.Among the fungal genera,only the relative abundance of Neoascochyta(root,leaf)and Mortierella(root)in resistant pepper cultivars was slightly higher than that of the susceptible pepper cultivars.In addition,the analysis of fungal community trophic types through the Funguild database showed that the relative abundance of the fungal communities in pathotroph in the stems and leaves of susceptible pepper(Fudijian)was higher than that of resistant pepper(SJ-0948 and CM334).关键词
辣椒/疫病/抗性/内生微生物/生态位Key words
pepper/phytophthora blight/resistance characteristic/endophytic microbes/ecological niche分类
农业科技引用本文复制引用
蔡泽彦,张明星,周池,陶禹,杨莎,李鑫,李雪峰..辣椒疫病不同抗性品种内生微生物群落多样性、结构及功能分析[J].园艺学报,2025,52(2):322-336,15.基金项目
现代农业产业技术体系建设专项资助(CARS-23-A07) （CARS-23-A07）
湖南省农业科技创新资金项目(2022cx39) （2022cx39）
