园艺学报2025，Vol.52Issue(2)：337-348,12.DOI:10.16420/j.issn.0513-353x.2023-0929
金针菇PRX家族基因鉴定及其在子实体发育过程中的表达分析
Sequence Characterization of PRX Family and Expression Analysis in the Process of Fruiting Body Development in Flammulina filiformis
摘要
Abstract
To understand the sequence and expression characteristics of the PRX family genes in Flammulina filiformis,bioinformatics methods were used to screen and identify the PRX gene family sequences from the whole genome of F.filiformis.Subsequently,the members of the gene family were analyzed in terms of gene structure,sequence characterization,chromosomal localization,cis-acting elements,and expression patterns.The results showed that there were six PRX genes distributed on six different chromosomes and localized in different cellular locations.The evolutionary analysis showed that the six genes could be classified into five subfamilies,namely 1CysPRX,2CysPRX,PRXQ,PRXⅡ,and PRXⅤ.The analysis of the cis-acting elements showed that the six genes contained cis-acting elements related to the growth,development,abiotic stresses.Expression pattern analysis showed that members of the gene family had different expression in different developmental periods and tissues of F.filiformis.The expression of FfPRXQ1 and FfPRX Ⅴ were significantly higher in the pileus of maturation stage than that in other parts,and the expression of Ff1CysPRX,FfPRXⅡ,and FfPRX Ⅴ were higher in the stable region than the elongation region and transitional region of the stipe.The expression of FfPRXQ2 was significantly higher in the stipe than in the pileus,and the expression at different periods of cap development,showing a first increase and then a decrease.The above results indicated that the PRX family genes were involved in the developmental regulation of the fruiting-body of F.filiformis,and there were obvious differences in the expression patterns.关键词
金针菇/第Ⅲ类过氧化物酶/子实体发育/表达分析Key words
Flammulina filiformis/class Ⅲ peroxidase/fruiting-body development/expression analysis分类
农业科技引用本文复制引用
仝宗军,严俊杰,韩星,段昕莲,刘媛媛,林俊彬,甘颖,陈杰,谢宝贵,甘炳成..金针菇PRX家族基因鉴定及其在子实体发育过程中的表达分析[J].园艺学报,2025,52(2):337-348,12.基金项目
国家自然科学基金青年科学基金项目(32102458) （32102458）
中国农业科学院科技创新工程项目(34-IUA-06) （34-IUA-06）
四川省育种联合攻关项目(2023YZ001) （2023YZ001）
