园艺学报2025，Vol.52Issue(2)：365-379,15.DOI:10.16420/j.issn.0513-353x.2023-0970
中国古老月季'月月粉'等位基因不平衡分析与分子标记开发利用
Allele-specific Imbalance Analysis and Related Molecular Marker Development in Rosa chinensis'Old Blush'
摘要
Abstract
In this study,a comprehensive comparative analysis of the two haplotype genomes of Rosa chinensis'Old Blush'were conducted using RcOB_hapl and RcOB_hap2.The allele-specific imbalance analysis was conducted by bioinformatics methods,and the corresponding molecular markers were developed,providing a basis for the breeding of the cultivated rose.The results showed that two haplotype genomes had a good collinearity relationship,but the assembly integrity of RcOB_hap2 is better than RcOB_hapl.The heterozygosity of'Old Blush'rose was 3.48％,containing a large number of single nucleotide polymorphisms,fragment insertions or deletions and chromosomal variations.A total of 1 157 allele-specific protein function genes and 720 allele-specific expression genes were identified in the whole genome.GO annotation and KEGG enrichment analyses of all imbalanced alleles showed that they were mainly involved in the processes of metabolism and synthesis,plant growth and development,and environmental adaptation.Imbalanced alleles AP2,MBY4 and DSC1 were selected for developing molecular markers for floral organ development,aroma synthesis and immune response,respectively.Based on the results of the molecular markers within 183 materials found that the Hap2 AP2 allele had incomplete protein function and was strongly selected in cultivated roses with double flower;For the MBY4,its Hap2 allele showed lower expression and was adopted by most cultivated roses,that might lead cultivated rose emitting less benzenoid volatile;DSC1 s Hapl allele had lower expression and possible defect protein function,but it was selected in cultivated varieties,that might result in a reduction of the disease resistance in cultivated roses.关键词
月季/等位基因不平衡/等位基因蛋白功能差异/等位基因差异表达/分子标记Key words
rose/allele-specific imbalance/allelic protein function differences/allele-specific expression/molecular marker分类
农业科技引用本文复制引用
阎旭,李月,傅小鹏,宁国贵,梁梅..中国古老月季'月月粉'等位基因不平衡分析与分子标记开发利用[J].园艺学报,2025,52(2):365-379,15.基金项目
国家自然科学基金青年科学基金项目(32302596) （32302596）
