园艺学报2025，Vol.52Issue(2)：380-394,15.DOI:10.16420/j.issn.0513-353x.2024-0158
常绿杜鹃种质遗传多样性分析及SSR指纹图谱构建
Genetic Diversity Analysis and SSR Fingerprint Construction of Evergreen Rhododendron Germplasms
摘要
Abstract
In this study,based on genomic sequencing data of Rhododendron delavayi Franch.,genomic-SSR distribution characteristics were analyzed and polymorphic SSR primers were developed.Furthermore,the 61 evergreen Rhododendron germplasms were analyzed for genetic relationship and fingerprint construction to realize their genetic relationships and speculate their possible parents.In the results,15 pairs of SSR primers with high polymorphism,good stability and strong intergeneric versatility were developed,and a total of 126 alleles were detected in 61 evergreen Rhododendron germplasms.The average number of alleles(Na)and the average effective number of alleles(Ne)were 8.400 and 3.4681,respectively.The mean observed heterozygosity(Ho)and expected heterozygosity(He)were 0.3421 and 0.5347,respectively,and the mean polymorphism information content(PIC)was 0.5163.According to the results of population genetic structure analysis,61 evergreen Rhododendron germplasms were divided into 3 subgroups,which were basically consistent with the results of phylogenetic analysis.Combined with the pedigree information,it was inferred that the ancestral parents of the introduced Japanese cultivars came from two different distribution areas,and all cultivars,including the self-bred cultivars,were the hybrids of the parental species from sect.Ponticum in subgen.Hymenanthes.Five SSR markers with high discriminability were used to construct the fingerprint of 61 evergreen Rhododendron germplasms successfully.关键词
常绿杜鹃/基因组SSR标记/遗传多样性/指纹图谱Key words
evergreen rhododendron/genomic SSR markers/genetic diversity/fingerprints分类
农业科技引用本文复制引用
张露,张平萍,解玮佳,许凤,彭绿春,宋杰,杜光辉,李世峰..常绿杜鹃种质遗传多样性分析及SSR指纹图谱构建[J].园艺学报,2025,52(2):380-394,15.基金项目
云南省科技人才与平台计划项目(202105AC160017) （202105AC160017）
云南省重大科技计划专项(202302AE090018) （202302AE090018）
云南省农业联合重点项目(202101BD070007-010) （202101BD070007-010）
