园艺学报2025，Vol.52Issue(2)：423-438,16.DOI:10.16420/j.issn.0513-353x.2024-0348
丛枝菌根真菌对盐胁迫下核桃幼苗生长及生理的影响
The Effect of Arbuscular Mycorrhizal Fungi on the Growth and Physiological Characteristics of Walnut Seedlings Under Salt Stress
摘要
Abstract
In order to investigate the effect and regulatory mechanisms of walnut seedlings'salt tolerance influenced by arbuscular mycorrhizal fungi(AMF),in this study,Juglans regia'Wen 185'seedings were used as experimental material.Pot experiments were conducted in a greenhouse where seedlings were inoculated with Funneliformis mosseae and Rhizophagus irregularis.The effects of these two AMFs on photosynthetic parameters,chlorophyll fluorescence,stomatal characteristics,antioxidant enzyme activity,and endogenous hormone levels were analyzed under different NaCl concentrations(0,100,200,and 300 mmol·L-1).Under NaCl stress,seedling growth and development were inhibited,photosynthetic performance decreased,and the activities of antioxidant enzymes(SOD,POD,APX)and proline(Pro)content increased.Seedlings inoculated with AMF exhibited significant increases in chlorophyll content,photosynthetic parameters,and levels of endogenous hormones(IAA,ABA,GA3,ZR),but the effects on the fluorescence intensity of the OJIP curve(I-P)and the efficiency of electron transport(ABS/RC,TRo/RC,ETo/RC)were not significant.AMF inoculation reduced the accumulation of hydrogen peroxide(H2O2)and malondialdehyde(MDA),mitigated oxidative stress,and improved stomatal width at 100 mmol·L-1 NaCl.At 300 mmol·L-1 NaCl,inoculation with F.mosseae enhanced antioxidant enzyme activity and Pro content.Principal component analysis showed that AMF mainly improved the salt tolerance of'Wen 185'walnut seedlings by promoting chlorophyll synthesis,enhancing photosynthetic performance,and increasing POD activity and Pro content,with the inoculation of F mosseae being more effective.关键词
核桃/丛枝菌根真菌/光合特性/生理生化/耐盐性Key words
walnut/arbuscular mycorrhizal fungi/photosynthetic characteristics/physiology and biochemistry/salt tolerance分类
农业科技引用本文复制引用
李敖,郑旭,吴承勖,聂瑞宁,姬新颖,唐佳莉,张俊佩..丛枝菌根真菌对盐胁迫下核桃幼苗生长及生理的影响[J].园艺学报,2025,52(2):423-438,16.基金项目
国家重点研发计划课题(2022YFD2200402) （2022YFD2200402）
