现代畜牧科技Issue(3)：1-4,4.DOI:10.19369/j.cnki.2095-9737.2025.03.001
猪源奇异变形杆菌分离鉴定、药敏试验和致病性试验
Isolation,Identification,Drug Sensitivity Test and Pathogenicity Test of Proteus mirabilis from Pig
摘要
Abstract
In this study,diseased pig tissue was taken as the research object,liver and intestinal contents of dead pigs were collected aseptically for bacteria isolation and purification culture,a strain of bacteria was isolated from liver tissue,and the isolated pathogens were identified and analyzed by biochemical test,16S rRNA PCR amplification and sequencing test,drug sensitivity test,pathogenicity test and other methods.Phylogenetic tree analysis showed that the homology with HQ169118.1 was the highest(99.93%).The identification results showed that this strain was P.mirabilis of porcine origin.The results of pathogenicity test showed that the isolated strain had strong pathogenicity.The immunoprotection test showed that the isolated strain had good immunogenicity.Drug sensitivity tests showed that the strain was sensitive to many drugs.The results enrich the epidemiological data of porcine Proteus mirabilis and provide reference for the prevention and control of the disease.关键词
奇异变形杆菌/病原分离鉴定/药敏试验/致病性Key words
P.mirabilis/isolation and identification of pathogen/drug susceptible test/pathogenicity分类
畜牧业引用本文复制引用
王欢,王振,张艳,薛沾枚,金振华,王丽坤,林秀蔚,王若丁,白长胜,尹珺伊,田秋丰,张军,王岩,刘秋瑾,沈思思,曹禹..猪源奇异变形杆菌分离鉴定、药敏试验和致病性试验[J].现代畜牧科技,2025,(3):1-4,4.基金项目
黑龙江省农业科学院畜牧兽医分院分院自拟项目(ZNKT-202214,CNYGG-2024015) （ZNKT-202214,CNYGG-2024015）
