现代畜牧科技Issue(3)：71-74,4.DOI:10.19369/j.cnki.2095-9737.2025.03.018
基于Cytb基因的牦牛肉种来源鉴定与应用
Identification of Yak Breed Origin Based on the Cytb Gene and Application
摘要
Abstract
In order to establish a rapid and accurate genetic identification method for yak,yellow cattle,and buffalo at the genetic level,for the source tracking and species identification of yak meat products.Using yak,yellow cattle,and buffalo as research materials,a pair of universal primers were used to amplify a conserved region of the Cytb gene in bovine mitochondria.PCR RFLP detection revealed three specific cleavage sites,which can be used for identifying the origin of mixed beef products.Using this method to detect yak meat products from different brands,and then verifying the accuracy of enzyme digestion results and the degree of contamination of counterfeit yak meat products from ordinary beef and water buffalo through sequencing.The results showed that three specific enzyme cleavage sites can be used for the identification of mixed beef products,and the phenomenon of using yellow cattle and water buffalo to impersonate yak meat products is more serious in the beef product market.关键词
牦牛/PCR-RFLP/Cytb基因/物种鉴定Key words
yak/PCR RFLP/Cytb gene/species identification分类
农业科技引用本文复制引用
何建文,党鹏举,金丽娜,韩建林..基于Cytb基因的牦牛肉种来源鉴定与应用[J].现代畜牧科技,2025,(3):71-74,4.基金项目
CAAS-ILRI联合研究项目:动物遗传资源的分子评价(2008-CI-03) （2008-CI-03）
甘肃省教育科技创新基金项目(2022B-360) （2022B-360）
