中国病理生理杂志2025，Vol.41Issue(3)：417-426,10.DOI:10.3969/j.issn.1000-4718.2025.03.001
ERα和ClC-3的周期性表达与他莫昔芬抗乳腺癌作用的相关性研究
Periodic expression of ERα and ClC-3 and their correlation with anti-breast cancer effect of tamoxifen
摘要
Abstract
AIM:This study aims to investigate of perodic expression,distribution and interaction between es-trogen receptor α(ERα)and ClC-3 chloride channel,and their relevance to the cell cycle specificity of tamoxifen(TAM)in anti-breast cancer treatment.METHODS:We utilized a web database to analyze the correlation between ERα and ClC-3 expression.Three-dimentional molecular simulation software and co-immunoprecipitation were employed to detect and analyze the interactions between these two proteins.To assess cell cycle dynamics,we performed thymidine(TdR)double-blocking release assay and used nocodazole to block the cell cycle,with subsequent analysis via flow cytometry.Cell viability was measured by MTT assay.Western blot was conducted to evaluate the protein expression levels of ERα and ClC-3,while immunofluorescence staining was utilized to assess their subcellular distribution.RESULTS:(1)Anal-ysis from the web database revealed a significant correlation between ERα and ClC-3 expression,and co-immunoprecipita-tion confirmed their interaction.(2)We successfully obtained human breast cancer T47D cells at different cycle stages us-ing the TdR double-blocking release method and nocodazole treatment.(3)Treatment with TAM primarily inhibited T47D cell viability during G2/M phase.(4)Both ERα and ClC-3 exhibited cyclic variations in protein expression,with their sub-cellular distributions showing periodicity and co-localization.(5)Protein interactions between ERα and ClC-3 were ob-served across all cell cycle phases;(6)After TAM treatment,ERα expression peaked in G2/M phase,while ClC-3 expres-sion remained unaffected.CONCLUSION:Our findings demonstrate cyclic differences in the expression and distribution of ERα and ClC-3 in human breast cancer T47D cells,along with confirmed interactions between these two proteins.The cyclic properties of ERα may play a role in mediating the cell cycle specificity of TAM's anti-breast cancer effect.关键词
乳腺癌/雌激素受体α/ClC-3氯通道/他莫昔芬/细胞周期Key words
breast cancer/estrogen receptor α/ClC-3 chloride channel/tamoxifen/cell cycle分类
临床医学引用本文复制引用
李雪苛,侯秀颖,刘世情,杨海峰,朱林燕,何伟丽..ERα和ClC-3的周期性表达与他莫昔芬抗乳腺癌作用的相关性研究[J].中国病理生理杂志,2025,41(3):417-426,10.基金项目
国家自然科学基金资助项目(No.81872133) （No.81872133）
