中国病理生理杂志2025，Vol.41Issue(3)：427-432,6.DOI:10.3969/j.issn.1000-4718.2025.03.002
NHE1抑制剂EIPA在肝癌细胞中的作用及机制
Role and mechanism of NHE1 inhibitor EIPA in hepatocellular carcino-ma cells
摘要
Abstract
AIM:To investigate the effects of ethylisopropylamiloride(EIPA),an inhibitor of Na+/H+ex-changer 1(NHE1),on the proliferation,migration,invasion,cell cycle and apoptosis of hepatocellular carcinoma cells,and to explore its mechanisms.METHODS:The human hepatocellular carcinoma cell line Huh7 was cultured in vitro,and treated with different concentrations of EIPA.The mRNA and protein expression of NHE1 in Huh7 cells was assessed by RT-qPCR and Western blot.The proliferation of Huh7 cells was examined by kFluor488-EdU staining.The migration and invasion of Huh7 cells were assessed by Transwell assays.The cell cycle distribution and apoptosis of these cells were detected by flow cytometry.Moreover,the intracellular pH was measured via the PTI high-speed ion imaging system.RE-SULTS:Treatment with EIPA significantly suppressed the mRNA and protein expression of NHE1(P＜0.01).It signifi-cantly inhibited the proliferation,migration and invasion of Huh7 cells(P＜0.05 or P＜0.01),promoted their apoptosis(P＜0.01),and caused significant S-phase arrest(P＜0.01).It also significantly reduced the intracellular pH of Huh7 cells.CONCLUSION:The NHE1 inhibitor EIPA regulates the intracellular pH by inhibiting the expression and function of NHE1,which consequently affects the proliferation,migration,invasion,cell cycle,and apoptosis of Huh7 cells.There-fore,NHE1 may be a potential target for the treatment of hepatocellular carcinoma.关键词
Na+/H+交换体1/乙基异丙基阿米洛利/肝细胞癌/肿瘤酸性微环境Key words
Na+/H+exchanger 1/ethylisopropylamiloride/hepatocellular carcinoma/acidic tumor microenvi-ronment分类
临床医学引用本文复制引用
金海,刘倩,李婧,赵思宇,朱加兴..NHE1抑制剂EIPA在肝癌细胞中的作用及机制[J].中国病理生理杂志,2025,41(3):427-432,6.基金项目
国家自然科学基金资助项目(No.82460466) （No.82460466）
贵州省科技计划项目(黔科合基础-ZK[2024]一般323号) （黔科合基础-ZK[2024]一般323号）
