中国病理生理杂志2025，Vol.41Issue(3)：444-452,9.DOI:10.3969/j.issn.1000-4718.2025.03.004
PCSK9通过MAPK/ERK通路促进卵巢癌细胞体外增殖和侵袭的实验研究
PCSK9 promotes proliferation and invasion of ovarian cancer cells in vi-tro through MAPK/ERK pathway
摘要
Abstract
AIM:This study aims to investigate the role and mechanism of proprotein convertase subtilisin/kexin type 9(PCSK9)in ovarian cancer.METHODS:We compared the expression levels of PCSK9 between ovarian cancer specimens and their corresponding adjacent non-cancerous tissues,while also assessing its expression in various ovarian cancer cell lines.Using a shRNA strategy,we reduced the expression of PCSK9 in ovarian cancer cell lines cul-tured in vitro,with confirmation via Western blot.The effects of PCSK9 downregulation on the proliferation,migration,and invasion of ovarian cancer cells were evaluated through EdU,colony formation,and Transwell assays.Additionally,we analyzed the impact of PCSK9 down-regulation on the MAPK/ERK signaling pathway using Western blot analysis.RE-SULTS:PCSK9 was significantly upregulated in ovarian cancer tissues and cell lines(P＜0.01).Downregulation of PC-SK9 resulted in a significant decrease in cell proliferation,migration,and invasion(P＜0.01).Western blot analysis dem-onstrated that PCSK9 knockdown led to reduced expression levels of key molecules within the MAPK/ERK signaling path-way(P＜0.01).CONCLUSION:PCSK9 promotes the proliferation and invasion of ovarian cancer cells by activating MAPK/ERK signaling pathway.关键词
卵巢癌/PCSK9蛋白/生物标志物/MAPK/ERK信号通路Key words
ovarian cancer/PCSK9 protein/biomarkers/MAPK/ERK signaling pathway分类
基础医学引用本文复制引用
吴敏敏,骆婕,廖凤儿,郑婷,范荻,郭琴..PCSK9通过MAPK/ERK通路促进卵巢癌细胞体外增殖和侵袭的实验研究[J].中国病理生理杂志,2025,41(3):444-452,9.基金项目
广东省医学科学技术研究基金项目(No.A2021477) （No.A2021477）
