中国病理生理杂志2025，Vol.41Issue(3)：472-480,9.DOI:10.3969/j.issn.1000-4718.2025.03.007
枳壳活性成分川陈皮素通过抑制心肌细胞焦亡减轻Ang Ⅱ诱导的心脏肥大
Nobiletin,an active ingredient of Fructus Aurantii,attenuates Ang Ⅱ-in-duced cardiac hypertrophy by inhibiting cardiomyocyte pyroptosis
摘要
Abstract
AIM:This study aimed to identify the key active components and signaling pathways in the tradi-tional Chinese medicine Fructus Aurantii that contribute to the prevention and treatment of cardiac hypertrophy,along with experimental validation.METHODS:H9C2 cardiomyocytes were pretreated with nobiletin(NOB)for 1 h and then ex-posed to 100 nmol/L angiotensin Ⅱ(Ang Ⅱ)for 24 h.RT-qPCR was used to quantify the mRNA expression of hypertrophy-related genes,including atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP)and myosin heavy chain 7(MYH7).Immunofluorescence staining was employed to assess the surface area of cardiomyocytes.Additionally,a kit was utilized to measure levels of pyroptosis-related factors such as lactate dehydrogenase(LDH),interleukin-1β(IL-1β),IL-18 and caspase-1,while Western blot was performed to evaluate the expression of gasdermin D and caspase-1.RESULTS:Network pharmacology analyses indicated that NOB is the key active component in Fructus Aurantii that regu-lates cardiac hypertrophy,potentially through the pyroptosis pathway.Further molecular biology experiments confirmed that NOB inhibits Ang Ⅱ-induced cardiac hypertrophy and pyroptosis.Furthermore,the involvement of the pyroptosis pathway was highlighted in the protective effects of NOB against cardiac hypertrophy.CONCLUSION:The active compo-nent NOB in the traditional Chinese medicine Fructus Aurantii alleviates cardiac hypertrophy by inhibiting pyroptosis.关键词
枳壳/川陈皮素/心脏肥大/细胞焦亡Key words
Fructus Aurantii/nobiletin/cardiac hypertrophy/pyroptosis分类
药学引用本文复制引用
谭敏,陈靖,曾艺,陈嘉钰,王宪庆,黄珊,蔡轶..枳壳活性成分川陈皮素通过抑制心肌细胞焦亡减轻Ang Ⅱ诱导的心脏肥大[J].中国病理生理杂志,2025,41(3):472-480,9.基金项目
国家自然科学基金资助项目(No.81300085) （No.81300085）
广东省医学科学技术研究基金资助项目(No.A2022063) （No.A2022063）
