中国病理生理杂志2025，Vol.41Issue(3)：492-500,9.DOI:10.3969/j.issn.1000-4718.2025.03.009
基于网络药理学及动物实验探究白香丹胶囊对大鼠焦虑样行为的作用及其机制
Effect of Baixiangdan capsule on anxiety-like behavior in rats and its mechanism explored via network pharmacology and animal experiments
摘要
Abstract
AIM:This study aimed to utilize network pharmacology and an anxiety rat model to investigate the intervention targets and underlying mechanisms of Baixiangdan capsule(BXD)in the treatment of anxiety-like behavior.METHODS:We screened the action targets of BXD on the anxiety model using network pharmacology,followed by Gene Ontology(GO)enrichment and the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of the identified targets.Additionally,we evaluated the effects of BXD on rat anxiety behaviors induced by foot shock,with measurement of serum level of c-Jun N-terminal kinase inhibitor(JIK),and the protein levels of c-Jun N-terminal kinase(JNK),phos-phorylated JNK(p-JNK)and JIK in the left hippocampus and hypothalamus.RESULTS:A total of 97 targets,including the dopamine D1/D3 receptors,were identified as intersection targets.GO enrichment analysis indicated that target pro-teins were significantly involved in molecular functions such as catecholamine binding,receptor activation,and signal transduction.KEGG pathway analysis revealed that neural receptor-ligand pathways,including dopaminergic synapses(15%)and neuroactive ligand-receptor interaction(18%),were notably represented.In behavioral experiments,BXD and diazepam treatments significantly reduced total path in the open-field test(P＜0.01),while the number of entries into the central region increased(P＜0.05).Additionally,the percentage of entry times of the open arm and the percentage of time spent in the open arm were also increased(P＜0.01).Furthermore,BXD treatment led to decreased ratio of p-JNK/JNK and increased level of JIK(P＜0.05).CONCLUSION:The traditional Chinese medicine BXD demonstrates a promising efficacy in alleviating anxiety-like behaviors in model rats.It operates through a multi-component,multi-target,and multi-pathway approach,primarily by modulating the JIK/JNK signaling pathway.关键词
白香丹胶囊/焦虑/c-Jun氨基末端激酶/c-Jun氨基末端激酶抑制因子/网络药理学Key words
Baixiangdan capsule/anxiety/c-Jun N-terminal kinase/c-Jun N-terminal kinase inhibitor/net-work pharmacology分类
中医学引用本文复制引用
曲嵩霖,穆晓飞,刘艳芬,李梦霞,郭英慧..基于网络药理学及动物实验探究白香丹胶囊对大鼠焦虑样行为的作用及其机制[J].中国病理生理杂志,2025,41(3):492-500,9.基金项目
国家自然科学基金面上项目(No.82074293) （No.82074293）
国家自然科学基金青年项目(No.81202616) （No.81202616）
山东省研究生教育教学改革研究项目(No.SDYJSJGC2024063) （No.SDYJSJGC2024063）
山东省研究生精品和优质专业学位教学案例库项目(No.SDYAL2024147) （No.SDYAL2024147）
