中国草地学报2025，Vol.47Issue(3)：74-81,8.DOI:10.16742/j.zgcdxb.20240135
基于SSR标记的67个苜蓿品种遗传多样性及群体结构分析
Genetic Diversity and Population Structure Analysis of 67 Alfalfa Cultivars Based on SSR Markers
摘要
Abstract
In this study,the genetic diversity and population genetic structure of 67 domestic and for-eign alfalfa cultivars were analyzed based on SSR molecular markers to explored the genetic relationship of alfalfa germplasm resources,in order to provide a theoretical basis for the identification of alfalfa cultivars and breeding of new cultivars.The results showed that 180 polymorphic bands were amplified with 15 pairs of primers,with a polymorphic site percentage of 96.33%.The average values of polymorphism informa-tion content,Shannon's genetic diversity information index and Nei's gene diversity index were 0.73,0.38 and 0.24,indicating the selected primers had high polymorphism.The 67 cultivars were divided into four groups by UPGMA cluster analysis,and there was no significant correlation between the cluster results and the geographical origin of the cultivar.The population genetic structure analysis based on Structure soft-ware divided 67 cultivars into 2 subgroups and 1 mixed population,which was consistent with the results of principal coordinate analysis(PCoA),indicating that most cultivars(85.07%)showed a single genetic background,while the mixed population exhibited gene penetration and had a more complex genetic back-ground.关键词
苜蓿/遗传多样性/群体遗传结构/SSR标记Key words
Alfalfa/Genetic diversity/Population genetic structure/SSR markers分类
农业科技引用本文复制引用
赵凌平,刘佳皓,杨智博,孙平..基于SSR标记的67个苜蓿品种遗传多样性及群体结构分析[J].中国草地学报,2025,47(3):74-81,8.基金项目
青海省重点研发与转化计划子课题(20191213) （20191213）
河南省科技攻关项目(252102111112) （252102111112）
河南科技大学博士启动基金项目(09001634) （09001634）
