中国草地学报2025，Vol.47Issue(3)：82-93,12.DOI:10.16742/j.zgcdxb.20240090
荒漠草原典型牧草根际、根内功能细菌的筛选及促生功能解析
Screening and Characterization of Growth-Promoting Bacteria from the Rhizosphere and Roots of Forage Grasses in Desert Steppe
摘要
Abstract
Functional microorganisms are essential for promoting plant growth in the desert steppe eco-system of Inner Mongolia,offering valuable theoretical support and a scientific foundation for grassland eco-logical restoration and sustainable management.However,comprehensive research on the species diversity and plant growth-promoting mechanisms of these microorganisms within the rhizosphere and endosphere of forage grasses in this region remains limited.This study employed high-throughput sequencing technology to provide a preliminarily analysis of the rhizosphere bacterial community composition associated with 12 dominant forage grass species in the desert steppe.Five bacterial strains(A1-A3 and B1-B2,respectively)were isolated from endosphere and rhizosphere and screened using nutrient-modified selective media based on the soil nutrient conditions of the experimental site.These strains were identified as Bacillus sp.(B1 and B2),Promicromonospora sp.(A1),and Streptomyces sp.(A2 and A3).Functional characterization revealed these strains exhibited strong nutrient-solubilizing capabilities.Strain A2 demonstrated a soluble phosphorus content of 164.51 mg/L in inorganic phosphorus fermentation broth,while strain A3 showed a soluble phosphorus content of 17.43 mg/L in organic phosphorus fermentation broth.All strains exhibited significant potassium-solubilizing activity.Furthermore,they produced plant growth-promoting hormones and displayed ACC deaminase activity.Strain B1 produced auxin at 26.08 μg/L.ACC deaminase activity ranged from 32.20 to 40.05 U/L for strains A1,A2,A3,and B1.All five strains promoted the growth of Neopallasia pectinata seedlings,particularly in enhancing radicle growth.关键词
荒漠草原/高通量测序/功能微生物/代谢产物/促生Key words
Desert steppe/High-throughput sequencing/Functional microorganisms/Metabolites/Growth promotion分类
农业科技引用本文复制引用
黄真真,高卉,李海港..荒漠草原典型牧草根际、根内功能细菌的筛选及促生功能解析[J].中国草地学报,2025,47(3):82-93,12.基金项目
内蒙古自然科学基金项目(2023QN03001) （2023QN03001）
内蒙古自治区科技计划(2021GG0076) （2021GG0076）
