中国草地学报2025，Vol.47Issue(3)：94-102,9.DOI:10.16742/j.zgcdxb.20240183
燕麦穗腐病病原菌鉴定及生物学特性分析
Identification and Biological Characteristics Analysis of the Pathogen Causing Spikelet Rot of Oat
摘要
Abstract
To clarify the pathogen species and their biological characteristics of oat spikelet rot in Guide county,Xining city,Qinghai province and Shandan county,Zhangye city,Gansu province,this study employed conventional tissue isolation methods to isolate pathogens,and used plate dilution method for single spore purification of isolates.According to Koch's postulates,combined with morphological and molecular biology techniques,58 isolates were identified as Dactylobotrys graminicola.The results of bio-logical characteristic analysis showed that the temperature range of mycelial growth of D.graminicola was 10-30℃,with the optimal temperature for growth and sporulation being 15℃.pH value had no significant effect on mycelial growth,and alkaline environment was more suitable for spore production.Complete darkness signicantly promoted the growth and sporulation of D.graminicola.D.graminicola could utilize various carbon and nitrogen sources,with inositol and ammonium chloride being the most suitable for the mycelial growth and sporulation,respectively.The results of this study provide a theoretical basis for the diagnosis and scientific prevention and control of oat spikelet rot.关键词
燕麦/禾生指葡孢霉/分离/鉴定/生物学特性Key words
Oat/Dactylobotrys graminicola/Isolation/Identification/Biological characteristics分类
农业科技引用本文复制引用
贾瑞芳,王娜,王乐,陈正强,林克剑,张园园..燕麦穗腐病病原菌鉴定及生物学特性分析[J].中国草地学报,2025,47(3):94-102,9.基金项目
国家重点研发计划"栽培草地病虫草害发生与为害机理及绿色综合防控技术"(2022YFD1300802) （2022YFD1300802）
中央级公益性科研院所基本科研业务费专项(1610332022003) （1610332022003）
内蒙古自治区自然科学基金项目(2024QN03080) （2024QN03080）
