中国草食动物科学2025，Vol.45Issue(2)：8-16,9.DOI:10.3969/j.issn.2095-3887.2024.00.122
围产后期影响牦牛产后恢复关键血液蛋白的筛选
Screening of Key Blood Proteins Affecting Postpartum Recovery in Yaks During the Late Perinatal Period
摘要
Abstract
Yak is one of the endemic animals in the Qinghai Tibet Plateau region,but its reproductive rate is consis-tently low,and the delayed recovery of their reproductive system during the late perinatal period is one of the main rea-sons.This study screened key proteins that affect postpartum recovery in yak during the late perinatal period using pro-teomics techniques,aiming to explore the mechanism of delayed postpartum recovery in yak during the late perinatal period.Forty multiparous yaks from Qinghai region were selected for the experiment.Blood samples were collected e-very 7 days from the date of delivery and divided into 5 groups based on time points:group A(delivery day),group B(7 days postpartum),group C(14 days postpartum),group D(21 days postpartum),and group E(28 days postpartum).Pro-teomic technology based on data independent scanning mode,protein mass spectra of each group was screened and compared pairwise.As a result,399 differentially expressed proteins were screened.And based on bioinformatics analy-sis,18 key proteins were identified,which were related to ovarian reproductive hormone secretion(PLA2,PKA,14-3-3,IGF1,IGFBP1,IGFBP4,IGFBP6,and IGFBP7),ovarian glucose metabolism and lipid metabolism(PFK,FBP,GAPDH,PK,LDHA,6PGDH,Apo IV,and LPL),as well as the other two key proteins(CBG and HSP90β).A network mechanism was constructed to regulate the reproductive system of yak postpartum key proteins.Through the analysis of key differentially expressed proteins,it is reflected that the abnormal secretion and metabolism of reproductive hor-mones in the ovaries of yaks after delivery,as well as the abnormal supply of energy from sugar metabolism and lipid metabolism,are the main reasons for the delayed recovery of the yak reproductive system after delivery.关键词
牦牛/产后恢复/围产期/蛋白质组学Key words
yak/postpartum recovery/perinatal period/proteomics分类
畜牧业引用本文复制引用
舒适,谢璐瑶,王国文,付长其,黄荣,关凯文,彭巍..围产后期影响牦牛产后恢复关键血液蛋白的筛选[J].中国草食动物科学,2025,45(2):8-16,9.基金项目
青海省科技成果转化专项项目(2022-NK-110) （2022-NK-110）
青海省科技特派员专项(2024-NK-P26) （2024-NK-P26）
