中国草食动物科学2025，Vol.45Issue(2)：17-24,8.DOI:10.3969/j.issn.2095-3887.2024.00.117
塔里木马鹿WNT5B基因序列特征及其在鹿茸组织中的表达特征
Sequence Characterization of WNT5B Gene and Its Expression Profile in Antler Tissues of Tarim Red Deer
摘要
Abstract
In order to investigate the role of WNT5B gene in the regeneration and development of Tarim Red deer antlers,this experiment was conducted using Tarim Red deer antlers as the test material,the sequence of CDS region of WNT5B gene was cloned,and the WNT5B protein of Tarim Red deer was expressed by using the recombinant technology of Escherichia coli and the sequence was analyzed by using bioinformatic methods.The expression changes in different tissues of antlers were detected by real-time fluorescence quantitative PCR and immunofluorescence.The results showed that the CDS region of the WNT5B gene of Tarim Red deer was 1 074 bp long,encoding 358 amino acids,with 100%amino acid sequence similarity to Cervus elaphus and Cervus canadensis,and 99.4%similarity to cattle and White-tailed deer.The secondary structure of the WNT5B protein of the Tarim Red deer was mainly based on the α-helix and irregular coiling.Under the induction temperature of 37℃and IPTG concentration of 1 mM,the WNT5B protein of Tarima Red deer antler was successfully expressed within 3-48 hours,with a molecular weight of approximately 40 kDa.The WNT5B gene was expressed in the antler velvet skin,mesenchyme,cartilage and bone of the Tarim Red deer antlers,in which the highest expression was found in the bone tissues,which was significantly higher than that in the other tissues(P<0.01),followed by that of cartilage,which was significantly higher than that of velvet skin and mesenchyme.In summary,the sequence of the CDS region of WNT5B gene in Tarim Red deer was successfully obtained,and the expression characteristics of WNT5B in different tissues of antler were determined.关键词
塔里木马鹿/鹿茸/WNT5B基因/分子克隆/免疫荧光/原核表达Key words
Tarim Red deer/antler/WNT5B gene/molecular cloning/immunofluorescence/prokaryotic expression分类
畜牧业引用本文复制引用
向欣,杨晨晨,林川,陈红,吕皓,韩春梅..塔里木马鹿WNT5B基因序列特征及其在鹿茸组织中的表达特征[J].中国草食动物科学,2025,45(2):17-24,8.基金项目
国家自然科学基金项目(31860629) （31860629）
塔里木大学研究生科研创新项目(TDGRI202336) （TDGRI202336）
