中国草食动物科学2025，Vol.45Issue(2)：58-64,7.DOI:10.3969/j.issn.2095-3887.2024.00.130
犊牛腹泻粪源弗格森埃希菌的分离鉴定及生物学特性研究
Isolation,Identification,and Its Biological Properties of Escherichia Fergusonii Originating from Calf Feces with Diarrhea
摘要
Abstract
In order to isolate and identify the causative organisms that cause diarrhoea in calves,and to grasp their drug resistance,19 samples of diarrhoeic calves(19)were collected from a large-scale cattle farm in Emin county,Xinjiang,and were tested.Escherichia fergusonii was isolated and identified by bacteriological culture and molecular biology techniques,and was subsequently tested for the presence of ultra-broad-spectrum β-lactamases(ESBLs)phenotypes,drug resistance and virulence genes.It was also tested for adhesion and invasion by mouse macrophages.The results in-dicated that two strains of E.fergusonii were isolated from 19 faecal samples(designation 292-1 and 337-1),both of which were identified as ESBLs-producing E.fergusonii with the blaTEM genotype.Drug sensitivity tests conducted on 15 antibiotics revealed that the two isolates exhibited multi-drug resistant to penicillin,cefotaxime,rifampicin and florfenicol.The results of drug resistance gene testing showed that both strains of E.fergusonii carried the blaTEM,floR,tetA,cmlA and sul2 genes.Additionally,the results of virulence gene testing revealed that both strains possessed the yijP,iucD,ibeB,ompA and sodA virulence genes.The results of the cellular assay demonstrated an adhesion rate of 3.23%for 292-1 and 0.59%for 337-1,as well as an invasion rate of 0.12%and 0.06%,respectively.关键词
犊牛/弗格森埃希菌/耐药性/毒力基因/细胞黏附与侵袭Key words
calf/Escherichia fergusonii/drug resistance/virulence genes/cell adhesion and invasion分类
畜牧业引用本文复制引用
于月通,马雯萱,马志远,杨彬,朱婷婷,吴静,李静,齐萌..犊牛腹泻粪源弗格森埃希菌的分离鉴定及生物学特性研究[J].中国草食动物科学,2025,45(2):58-64,7.基金项目
新疆生产建设兵团科技创新人才计划(2021CB062) （2021CB062）
新疆生产建设兵团第九师科技计划项目(2022JS008) （2022JS008）
兵团指导性科技计划项目(2023ZD113) （2023ZD113）
