中国草食动物科学2025，Vol.45Issue(2)：72-79,8.DOI:10.3969/j.issn.2095-3887.2024.00.138
牦牛TMEM182基因克隆及组织表达
Cloning and Tissue Expression Analysis of TMEM182 Gene in Yak
摘要
Abstract
To elucidate the biological structure and function of the TMEM182 gene in yaks,as well as its expression profile across various tissues,this study utilized cDNA derived from the muscle tissue of domestic yaks to clone the CDS region of the TMEM182 gene.Subsequently,a comprehensive bioinformatics assessment was carried out.Finally,the expression levels of the TMEM182 gene were determined in lung,kidney,heart,liver,muscle,spleen,testis,and adi-pose tissue of yak.The results revealed that The CDS region sequence length of the TMEM182 gene in domestic yak is 690 bp,encoding 299 amino acids.Nucleotide sequence alignment indicated that homology between domestic yak and wild yak was the highest at 99.7%,while it was the lowest with macaques at 85.9%.Bioinformatics analysis addition-ally revealed that the theoretical isoelectric point of the TMEM182 protein in domestic yak is 6.48;it is characterized as a slightly acidic hydrophobic protein lacking a signal peptide but possessing transmembrane domains,and mainly dis-tributed in the endoplasmic reticulum.This protein has 12 potential phosphorylation sites and 4 glycosylation sites.The secondary structure mainly comprises random coils.TMEM182 protein interacts significantly with proteins such as TM6SF1,MCTP2,TSPAN33,and CDH15.Results from qRT-PCR indicated varying degrees of expression for the TMEM182 gene across all examined tissues in domestic yak,in which notably higher expression levels were observed in testis,muscle,and adipose tissues compared to other tissues(P<0.01).关键词
家牦牛/TMEM182基因/克隆/组织表达Key words
domestic yaks/TMEM182 gene/clone/tissue expression分类
畜牧业引用本文复制引用
张明昊,王佟,包鹏甲,黄纯,于沁冉,邓婧瑛,褚敏,阎萍..牦牛TMEM182基因克隆及组织表达[J].中国草食动物科学,2025,45(2):72-79,8.基金项目
甘肃省科技重大专项计划(21ZD10NA001,GZGG-2021-1) （21ZD10NA001,GZGG-2021-1）
深圳市科技计划项目(KCXF20201221173205015) （KCXF20201221173205015）
新疆天池英才(TCYC-TP2023) （TCYC-TP2023）
昌吉州庭州英才(TZYC-2023) （TZYC-2023）
