中国肛肠病杂志2025，Vol.45Issue(2)：8-12,5.
基于网络药理学探讨葛根芩连汤治疗溃疡性结肠炎的分子靶点及作用机制
Molecular Target and Mechanism of Gegen Qinlian Decoction in Treating Ulcerative Colitis Based on Network Pharmacology
摘要
Abstract
Objective To explore the molecular target of Gegen Qinlian Decoction in the treatment of ul-cerative colitis(UC)and its mechanism in the treatment of UC through network pharmacology.Methods The active components of Gegen(Puerariae Lobatae Radix),Huangqin(Scutellariae Radix),Huanglian(Coptidis Rhizoma),Gancao(Glycyrrhizae Radix et Rhizoma)were screened using TCMSP.The molecular targets were identified in UniProt database,and the disease genes were obtained in DisGeNET database to get the intersections by comparison.Cross-gene was analyzed by protein-protein interaction(PPI)con-struction,GO functional enrichment analysis and KEGG pathway enrichment analysis.The common targets were imported into the String database,and the top 30 targets in the interaction relationship were obtained through the interaction diagram of the core targets obtained after processing by Cytoscape software.GO functional enrichment analysis was carried out on the common targets of Gegen Qinlian Decoction and UC.Enrichment was carried out from three levels:biological process(BP),cell component(CC)and molecular function(MF).KEGG path enrichment analysis was performed using R software.Results STAT3,RE-LA,TNF,IL6,MAPK3,MAPK14,TP53,AKT1,MAPK1,STAT1 and other genes ranked high and were the core targets.BP mainly involved the reaction to lipopolysaccharide,the reaction to bacteria-derived mol-ecules,the reaction to oxidative stress,and the process of active oxygen metabolism.CC was mainly related to the raft structure of eukaryotic cell membrane,membrane microdomain,membrane binding organelle structure and plasma membrane cave-like invagination.MF was mainly involved in cytokine receptor bind-ing,cytokine activity,receptor ligand activity,and signaling receptor activator activity,etc.The pathways with a large number of enriched genes were AGE-RAGE signaling pathway in diabetic complications,lipid and atherosclerosis signaling pathway,and IL-17 signaling pathway,and some cancer pathways.Conclu-sion Gegen Qinlian Decoction may play a therapeutic role in UC through the above key targets and related signaling pathways in anti-inflammation,antioxidation and inhibition of oncogene transcription.关键词
葛根芩连汤/溃疡性结肠炎/分子靶点/作用机制/网络药理学Key words
Gegen Qinlian Decoction/Ulcerative colitis/Molecular target/Mechanism/Network pharma-cology引用本文复制引用
逄承健,王淑淑,乔翠霞,赵刚..基于网络药理学探讨葛根芩连汤治疗溃疡性结肠炎的分子靶点及作用机制[J].中国肛肠病杂志,2025,45(2):8-12,5.基金项目
山东省中医药科技项目(编号Q-2023192) （编号Q-2023192）
